Hypoxia induces glucose uptake and metabolism of adipose-derived stem cells

Hyoung Sook Park, Ji Hye Kim, Bo Kyung Sun, Sun U. Song, Wonhee Suh, Jong Hyuk Sung

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

It has previously been demonstrated that hypoxia has diverse stimulatory effects on adipose-derived stem cells (ASCs), however, metabolic responses under hypoxia remain to be elucidated. Thus, the present study aimed to investigate the glucose uptake and metabolism of ASCs under hypoxic conditions, and to identify the underlying molecular mechanisms. ASCs were cultured in 1% oxygen, and experiments were conducted in vitro. As determined by proteomic analysis and western blotting, GAPDH and enolase 1 (ENO1) expression were upregulated under hypoxia. In addition, lactate production was significantly increased, and mRNA levels of glycolytic enzymes, including GAPDH, ENO1, hexokinase 2 (HK2), and lactate dehydrogenase (LDH-) were upregulated. Hypoxia-inducible factor 1 (HIF-1-) expression was increased as demonstrated by western blotting, and a pharmacological inhibitor of HIF-1- significantly attenuated hypoxia-induced lactate production and expression of glycolytic enzymes. It was also observed that hypoxia significantly increased glucose uptake in ASCs, and glucose transporter (GLUT)1 and GLUT3 expression were upregulated under hypoxia. Pharmacological inhibition of the HIF-1- signaling pathways also attenuated hypoxia-induced GLUT1 and GLUT3 expression. These results collectively indicate that hypoxia increases glucose uptake via GLUT1 and GLUT3 upregulation, and induces lactate production of ASCs via GAPDH, ENO1, HK2, and LDH-. Furthermore, HIF-1- is involved in glucose uptake and metabolism of ASCs.

Original languageEnglish
Pages (from-to)4706-4714
Number of pages9
JournalMolecular Medicine Reports
Volume14
Issue number5
DOIs
Publication statusPublished - 2016 Nov

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Oncology
  • Cancer Research

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