Hypoxia-preconditioned adipose tissue-derived mesenchymal stem cell increase the survival and gene expression of engineered neural stem cells in a spinal cord injury model

Jin Soo Oh, Yoon Ha, Sung Su An, Momin Khan, William A. Pennant, Hyo Jin Kim, Do Heum Yoon, Minhyung Lee, Keung Nyun Kim

Research output: Contribution to journalArticle

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Abstract

Hypoxic preconditioning (HP) is a novel strategy to make stem cells resistant to the ischemic environment they encounter after transplantation into injured tissue; this strategy improves survival of both the transplanted cells and the host cells at the injury site. Using both in vitro and in vivo injury models, we confirmed that HP-treated adipose tissue-derived mesenchymal stem cells (HP-AT-MSCs) increased cell survival and enhanced the expression of marker genes in DsRed-engineered neural stem cells (NSCs-DsRed). Similar to untreated AT-MSCs, HP-AT-MSCs had normal morphology and were positive for the cell surface markers CD90, CD105, and CD29, but not CD31. In three in vitro ischemic-mimicking injury models, HP-AT-MSCs significantly increased both the viability of NSCs-DsRed and the expression of DsRed and clearly reduced the number of annexin-V-positive apoptotic NSCs-DsRed and the expression of the apoptotic factor Bax. Consistent with the in vitro assay, co-transplantation of NSCs-DsRed with HP-AT-MSCs significantly improved the survival of the NSCs-DsRed, resulting in an increased expression of the DsRed reporter gene at the transplantation site in a rat spinal cord injury (SCI) model. These findings suggest that the co-transplantation of HP-AT-MSCs with engineered NSCs can improve both the cell survival and the gene expression of the engineered NSCs, indicating that this novel strategy can be used to augment the therapeutic efficacy of combined stem cell and gene therapies for SCI.

Original languageEnglish
Pages (from-to)215-219
Number of pages5
JournalNeuroscience Letters
Volume472
Issue number3
DOIs
Publication statusPublished - 2010 Mar 26

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Neural Stem Cells
Mesenchymal Stromal Cells
Spinal Cord Injuries
Adipose Tissue
Cell Survival
Gene Expression
Transplantation
Wounds and Injuries
Stem Cells
fluorescent protein 583
Hypoxia
Annexin A5
Cell- and Tissue-Based Therapy
Reporter Genes
Genetic Therapy

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Oh, Jin Soo ; Ha, Yoon ; An, Sung Su ; Khan, Momin ; Pennant, William A. ; Kim, Hyo Jin ; Yoon, Do Heum ; Lee, Minhyung ; Kim, Keung Nyun. / Hypoxia-preconditioned adipose tissue-derived mesenchymal stem cell increase the survival and gene expression of engineered neural stem cells in a spinal cord injury model. In: Neuroscience Letters. 2010 ; Vol. 472, No. 3. pp. 215-219.
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Hypoxia-preconditioned adipose tissue-derived mesenchymal stem cell increase the survival and gene expression of engineered neural stem cells in a spinal cord injury model. / Oh, Jin Soo; Ha, Yoon; An, Sung Su; Khan, Momin; Pennant, William A.; Kim, Hyo Jin; Yoon, Do Heum; Lee, Minhyung; Kim, Keung Nyun.

In: Neuroscience Letters, Vol. 472, No. 3, 26.03.2010, p. 215-219.

Research output: Contribution to journalArticle

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AU - Oh, Jin Soo

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AU - An, Sung Su

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