IAA and N 6 -benzyladenine inhibit ethylene-regulated expression of ACC oxidase and ACC synthase genes in mungbean hypocotyls

Jeong Hoe Kim, Woo Taek Kim, Bin G. Kang

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Regulation of expression of 1-aminocyclopropane-1-carboxylate (ACC) oxidase (VR-ACO1) and ACC synthase (VR-ACS1) genes by ethylene, indole-3-acetic acid (IAA), and N 6 -benzyladenine (BA), was investigated in mungbean hypocotyl tissues. Exogenous ethylene markedly increased transcript level of VR-ACO1 and reduced that of VR-ACS1, whereas aminooxyacetic acid (AOA), an inhibitor of ethylene biosynthesis, decreased the level of VR-ACO1 mRNA and increased that of VR-ACS1, indicating that expression of VR-ACO1 and VR-ACS1 genes are under positive and negative feedback control by ethylene, respectively. However, IAA treatment reduced the level of VR-ACO1 transcripts and increased that of VR-ACS1, although the hormone greatly induced ethylene production. We have demonstrated that, in a system that separates the effect of IAA proper from the effect of IAA-induced ethylene, the amount of IAA-induced ethylene was enough to cause accumulation of VR-ACO1 mRNA and decrease of VR-ACS1 mRNA. We have also shown that the responsiveness of VR-ACO1 and VR-ACS1 to exogenous ethylene was greatly reduced in the presence of IAA. In addition, BA abolished ethylene responsiveness with respect to expression of VR-ACO1 and VR-ACS1. Based on these results, we suggest that IAA and BA inhibit ethylene action, resulting in suppression of VR-ACO1 expression and induction of VR-ACS1 expression.

Original languageEnglish
Pages (from-to)1056-1061
Number of pages6
JournalPlant and Cell Physiology
Volume42
Issue number10
DOIs
Publication statusPublished - 2001 Jan 1

Fingerprint

N-benzyladenine
1-aminocyclopropanecarboxylate synthase
1-aminocyclopropane-1-carboxylate synthase
Hypocotyl
mung beans
benzyladenine
indole acetic acid
hypocotyls
ethylene
Genes
genes
ethylene production
Messenger RNA
aminooxyacetic acid
indoleacetic acid
1-aminocyclopropane-1-carboxylic acid oxidase
Aminooxyacetic Acid
acid treatment

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

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title = "IAA and N 6 -benzyladenine inhibit ethylene-regulated expression of ACC oxidase and ACC synthase genes in mungbean hypocotyls",
abstract = "Regulation of expression of 1-aminocyclopropane-1-carboxylate (ACC) oxidase (VR-ACO1) and ACC synthase (VR-ACS1) genes by ethylene, indole-3-acetic acid (IAA), and N 6 -benzyladenine (BA), was investigated in mungbean hypocotyl tissues. Exogenous ethylene markedly increased transcript level of VR-ACO1 and reduced that of VR-ACS1, whereas aminooxyacetic acid (AOA), an inhibitor of ethylene biosynthesis, decreased the level of VR-ACO1 mRNA and increased that of VR-ACS1, indicating that expression of VR-ACO1 and VR-ACS1 genes are under positive and negative feedback control by ethylene, respectively. However, IAA treatment reduced the level of VR-ACO1 transcripts and increased that of VR-ACS1, although the hormone greatly induced ethylene production. We have demonstrated that, in a system that separates the effect of IAA proper from the effect of IAA-induced ethylene, the amount of IAA-induced ethylene was enough to cause accumulation of VR-ACO1 mRNA and decrease of VR-ACS1 mRNA. We have also shown that the responsiveness of VR-ACO1 and VR-ACS1 to exogenous ethylene was greatly reduced in the presence of IAA. In addition, BA abolished ethylene responsiveness with respect to expression of VR-ACO1 and VR-ACS1. Based on these results, we suggest that IAA and BA inhibit ethylene action, resulting in suppression of VR-ACO1 expression and induction of VR-ACS1 expression.",
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IAA and N 6 -benzyladenine inhibit ethylene-regulated expression of ACC oxidase and ACC synthase genes in mungbean hypocotyls . / Kim, Jeong Hoe; Kim, Woo Taek; Kang, Bin G.

In: Plant and Cell Physiology, Vol. 42, No. 10, 01.01.2001, p. 1056-1061.

Research output: Contribution to journalArticle

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T1 - IAA and N 6 -benzyladenine inhibit ethylene-regulated expression of ACC oxidase and ACC synthase genes in mungbean hypocotyls

AU - Kim, Jeong Hoe

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