IAA and N6-benzyladenine inhibit ethylene-regulated expression of ACC oxidase and ACC synthase genes in mungbean hypocotyls

Jeong Hoe Kim, Woo Taek Kim, Bin G. Kang

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34 Citations (Scopus)

Abstract

Regulation of expression of 1-aminocyclopropane-1-carboxylate (ACC) oxidase (VR-ACO1) and ACC synthase (VR-ACS1) genes by ethylene, indole-3-acetic acid (IAA), and N6-benzyladenine (BA), was investigated in mungbean hypocotyl tissues. Exogenous ethylene markedly increased transcript level of VR-ACO1 and reduced that of VR-ACS1, whereas aminooxyacetic acid (AOA), an inhibitor of ethylene biosynthesis, decreased the level of VR-ACO1 mRNA and increased that of VR-ACS1, indicating that expression of VR-ACO1 and VR-ACS1 genes are under positive and negative feedback control by ethylene, respectively. However, IAA treatment reduced the level of VR-ACO1 transcripts and increased that of VR-ACS1, although the hormone greatly induced ethylene production. We have demonstrated that, in a system that separates the effect of IAA proper from the effect of IAA-induced ethylene, the amount of IAA-induced ethylene was enough to cause accumulation of VR-ACO1 mRNA and decrease of VR-ACS1 mRNA. We have also shown that the responsiveness of VR-ACO1 and VR-ACS1 to exogenous ethylene was greatly reduced in the presence of IAA. In addition, BA abolished ethylene responsiveness with respect to expression of VR-ACO1 and VR-ACS1. Based on these results, we suggest that IAA and BA inhibit ethylene action, resulting in suppression of VR-ACO1 expression and induction of VR-ACS1 expression.

Original languageEnglish
Pages (from-to)1056-1061
Number of pages6
JournalPlant and Cell Physiology
Volume42
Issue number10
DOIs
Publication statusPublished - 2001

Bibliographical note

Funding Information:
This work was supported by Korea Research Foundation Grant (KRF-99–019-D00142) to B.G.K, and by MOST (Plant Diversity Research Center, PF 003105–01), KOSEF (Plant Metabolism Research Center), and Yonsei University (Faculty Research Project 2000) to W.T.K. We thank Dr. Hans Kende (Michigan State University, East Lansing) for critical reading of the manuscript.

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

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