Identification and characterization of adenovirus early region 1B-associated protein 5 as a surface marker on undifferentiated human embryonic stem cells

Hong Seo Choi, Won Tae Kim, Hana Kim, Jum Ji Kim, Ji Yun Ko, Sang Wang Lee, Young Joo Jang, Sang Jick Kim, Min Jung Lee, Han Sung Jung, Julia Kzhyshkowska, Soo Jong Um, Mi Young Lee, Sang Hun Lee, Cheorl Ho Kim, Chun Jeih Ryu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Pluripotent human embryonic stem cells (hESCs) provide appropriate systems for developmental studies and prospective donor cell sources for regenerative medicine. Identification of surface markers specific to hESCs is a prerequisite for studying hESC biology and can be used to generate clinical-level donor cell preparations that are free from tumorigenic undifferentiated hESCs. We previously reported the generation of monoclonal antibodies that specifically recognize hESC surface antigens using a decoy immunization strategy. In this study, we show that monoclonal antibody 57-C11 recognizes a phosphorylated form of adenovirus early region 1B-associated protein 5 (E1B-AP5). E1B-AP5 is a nuclear RNA-binding protein, but we report that 57-C11-reactive E1B-AP5 is expressed on the surface of undifferentiated hESCs. In undifferentiated hESCs, 57-C11-reactive E1B-AP5 is localized to SSEA3-, SSEA4-, TRA-1-60-, TRA-1-81-, OCT4-, SOX2-, and NANOG-positive hESCs. In mixtures of undifferentiated hESCs and hESC-derived neurons, 57-C11 exclusively recognizes undifferentiated hESCs but not hESC-derived neuronal cells. Further, the expression of 57-C11-reactive E1B-AP5 decreases upon differentiation. Our results demonstrate that 57-C11-reactive E1B-AP5 is a novel surface molecule that is involved in the undifferentiated state of hESCs. As far as we know, this is the first report demonstrating that heterogeneous nuclear RNA-binding protein is expressed on the surface of undifferentiated hESCs.

Original languageEnglish
Pages (from-to)609-620
Number of pages12
JournalStem Cells and Development
Volume20
Issue number4
DOIs
Publication statusPublished - 2011 Apr 1

Fingerprint

Adenoviridae
Proteins
RNA-Binding Proteins
Nuclear Proteins
Human Embryonic Stem Cells
Heterogeneous Nuclear RNA
Monoclonal Antibodies
Nuclear RNA
Regenerative Medicine
Surface Antigens
Cell Biology
Immunization
Prospective Studies

All Science Journal Classification (ASJC) codes

  • Hematology
  • Developmental Biology
  • Cell Biology

Cite this

Choi, Hong Seo ; Kim, Won Tae ; Kim, Hana ; Kim, Jum Ji ; Ko, Ji Yun ; Lee, Sang Wang ; Jang, Young Joo ; Kim, Sang Jick ; Lee, Min Jung ; Jung, Han Sung ; Kzhyshkowska, Julia ; Um, Soo Jong ; Lee, Mi Young ; Lee, Sang Hun ; Kim, Cheorl Ho ; Ryu, Chun Jeih. / Identification and characterization of adenovirus early region 1B-associated protein 5 as a surface marker on undifferentiated human embryonic stem cells. In: Stem Cells and Development. 2011 ; Vol. 20, No. 4. pp. 609-620.
@article{e485efafe51a45ca897819ca0bab6578,
title = "Identification and characterization of adenovirus early region 1B-associated protein 5 as a surface marker on undifferentiated human embryonic stem cells",
abstract = "Pluripotent human embryonic stem cells (hESCs) provide appropriate systems for developmental studies and prospective donor cell sources for regenerative medicine. Identification of surface markers specific to hESCs is a prerequisite for studying hESC biology and can be used to generate clinical-level donor cell preparations that are free from tumorigenic undifferentiated hESCs. We previously reported the generation of monoclonal antibodies that specifically recognize hESC surface antigens using a decoy immunization strategy. In this study, we show that monoclonal antibody 57-C11 recognizes a phosphorylated form of adenovirus early region 1B-associated protein 5 (E1B-AP5). E1B-AP5 is a nuclear RNA-binding protein, but we report that 57-C11-reactive E1B-AP5 is expressed on the surface of undifferentiated hESCs. In undifferentiated hESCs, 57-C11-reactive E1B-AP5 is localized to SSEA3-, SSEA4-, TRA-1-60-, TRA-1-81-, OCT4-, SOX2-, and NANOG-positive hESCs. In mixtures of undifferentiated hESCs and hESC-derived neurons, 57-C11 exclusively recognizes undifferentiated hESCs but not hESC-derived neuronal cells. Further, the expression of 57-C11-reactive E1B-AP5 decreases upon differentiation. Our results demonstrate that 57-C11-reactive E1B-AP5 is a novel surface molecule that is involved in the undifferentiated state of hESCs. As far as we know, this is the first report demonstrating that heterogeneous nuclear RNA-binding protein is expressed on the surface of undifferentiated hESCs.",
author = "Choi, {Hong Seo} and Kim, {Won Tae} and Hana Kim and Kim, {Jum Ji} and Ko, {Ji Yun} and Lee, {Sang Wang} and Jang, {Young Joo} and Kim, {Sang Jick} and Lee, {Min Jung} and Jung, {Han Sung} and Julia Kzhyshkowska and Um, {Soo Jong} and Lee, {Mi Young} and Lee, {Sang Hun} and Kim, {Cheorl Ho} and Ryu, {Chun Jeih}",
year = "2011",
month = "4",
day = "1",
doi = "10.1089/scd.2010.0265",
language = "English",
volume = "20",
pages = "609--620",
journal = "Stem Cells and Development",
issn = "1547-3287",
publisher = "Mary Ann Liebert Inc.",
number = "4",

}

Choi, HS, Kim, WT, Kim, H, Kim, JJ, Ko, JY, Lee, SW, Jang, YJ, Kim, SJ, Lee, MJ, Jung, HS, Kzhyshkowska, J, Um, SJ, Lee, MY, Lee, SH, Kim, CH & Ryu, CJ 2011, 'Identification and characterization of adenovirus early region 1B-associated protein 5 as a surface marker on undifferentiated human embryonic stem cells', Stem Cells and Development, vol. 20, no. 4, pp. 609-620. https://doi.org/10.1089/scd.2010.0265

Identification and characterization of adenovirus early region 1B-associated protein 5 as a surface marker on undifferentiated human embryonic stem cells. / Choi, Hong Seo; Kim, Won Tae; Kim, Hana; Kim, Jum Ji; Ko, Ji Yun; Lee, Sang Wang; Jang, Young Joo; Kim, Sang Jick; Lee, Min Jung; Jung, Han Sung; Kzhyshkowska, Julia; Um, Soo Jong; Lee, Mi Young; Lee, Sang Hun; Kim, Cheorl Ho; Ryu, Chun Jeih.

In: Stem Cells and Development, Vol. 20, No. 4, 01.04.2011, p. 609-620.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification and characterization of adenovirus early region 1B-associated protein 5 as a surface marker on undifferentiated human embryonic stem cells

AU - Choi, Hong Seo

AU - Kim, Won Tae

AU - Kim, Hana

AU - Kim, Jum Ji

AU - Ko, Ji Yun

AU - Lee, Sang Wang

AU - Jang, Young Joo

AU - Kim, Sang Jick

AU - Lee, Min Jung

AU - Jung, Han Sung

AU - Kzhyshkowska, Julia

AU - Um, Soo Jong

AU - Lee, Mi Young

AU - Lee, Sang Hun

AU - Kim, Cheorl Ho

AU - Ryu, Chun Jeih

PY - 2011/4/1

Y1 - 2011/4/1

N2 - Pluripotent human embryonic stem cells (hESCs) provide appropriate systems for developmental studies and prospective donor cell sources for regenerative medicine. Identification of surface markers specific to hESCs is a prerequisite for studying hESC biology and can be used to generate clinical-level donor cell preparations that are free from tumorigenic undifferentiated hESCs. We previously reported the generation of monoclonal antibodies that specifically recognize hESC surface antigens using a decoy immunization strategy. In this study, we show that monoclonal antibody 57-C11 recognizes a phosphorylated form of adenovirus early region 1B-associated protein 5 (E1B-AP5). E1B-AP5 is a nuclear RNA-binding protein, but we report that 57-C11-reactive E1B-AP5 is expressed on the surface of undifferentiated hESCs. In undifferentiated hESCs, 57-C11-reactive E1B-AP5 is localized to SSEA3-, SSEA4-, TRA-1-60-, TRA-1-81-, OCT4-, SOX2-, and NANOG-positive hESCs. In mixtures of undifferentiated hESCs and hESC-derived neurons, 57-C11 exclusively recognizes undifferentiated hESCs but not hESC-derived neuronal cells. Further, the expression of 57-C11-reactive E1B-AP5 decreases upon differentiation. Our results demonstrate that 57-C11-reactive E1B-AP5 is a novel surface molecule that is involved in the undifferentiated state of hESCs. As far as we know, this is the first report demonstrating that heterogeneous nuclear RNA-binding protein is expressed on the surface of undifferentiated hESCs.

AB - Pluripotent human embryonic stem cells (hESCs) provide appropriate systems for developmental studies and prospective donor cell sources for regenerative medicine. Identification of surface markers specific to hESCs is a prerequisite for studying hESC biology and can be used to generate clinical-level donor cell preparations that are free from tumorigenic undifferentiated hESCs. We previously reported the generation of monoclonal antibodies that specifically recognize hESC surface antigens using a decoy immunization strategy. In this study, we show that monoclonal antibody 57-C11 recognizes a phosphorylated form of adenovirus early region 1B-associated protein 5 (E1B-AP5). E1B-AP5 is a nuclear RNA-binding protein, but we report that 57-C11-reactive E1B-AP5 is expressed on the surface of undifferentiated hESCs. In undifferentiated hESCs, 57-C11-reactive E1B-AP5 is localized to SSEA3-, SSEA4-, TRA-1-60-, TRA-1-81-, OCT4-, SOX2-, and NANOG-positive hESCs. In mixtures of undifferentiated hESCs and hESC-derived neurons, 57-C11 exclusively recognizes undifferentiated hESCs but not hESC-derived neuronal cells. Further, the expression of 57-C11-reactive E1B-AP5 decreases upon differentiation. Our results demonstrate that 57-C11-reactive E1B-AP5 is a novel surface molecule that is involved in the undifferentiated state of hESCs. As far as we know, this is the first report demonstrating that heterogeneous nuclear RNA-binding protein is expressed on the surface of undifferentiated hESCs.

UR - http://www.scopus.com/inward/record.url?scp=79953700592&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79953700592&partnerID=8YFLogxK

U2 - 10.1089/scd.2010.0265

DO - 10.1089/scd.2010.0265

M3 - Article

C2 - 21083500

AN - SCOPUS:79953700592

VL - 20

SP - 609

EP - 620

JO - Stem Cells and Development

JF - Stem Cells and Development

SN - 1547-3287

IS - 4

ER -