Identification and characterization of mutations in the high growth vaccine strain of influenza virus

K. H. Lee, J. W. Youn, H. J. Kim, Baik Lin Seong

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

X-31(H3N2) virus, which is a high yielding reassortant between A/PR/8/34(H1N1) and A/Aichi/68(H3N2), is currently used as a backbone strain for influenza vaccine production. The sequence of the current X-31 virus was determined from cloned cDNA of 6 internal RNA genes, and was compared with the original sequence of the A/PR/8/34 virus. 71 point mutations were accumulated in the six internal viral genes (PB2, PB1, PA, NP, M and NS). These nucleotide changes encode 23 amino acid substitutions in seven viral proteins (PB2, PB1, PA, M1, M2, NS1 and NS2). Among three polymerase genes, a significantly low mutation frequency was observed in PA gene as compared to PB2 and PB1. The mutation frequency at the nucleotide level was significantly low in NP gene without any amino acid substitution, being only about 20% of those observed in 5 other internal genes. The unequal distribution of mutations among different viral proteins may correlate with individual role of each protein in viral growth.

Original languageEnglish
Pages (from-to)369-377
Number of pages9
JournalArchives of Virology
Volume146
Issue number2
DOIs
Publication statusPublished - 2001 Apr 12

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Influenza Vaccines
Viral Proteins
Mutation
Growth
Genes
Mutation Rate
Amino Acid Substitution
Nucleotides
H3N2 Subtype Influenza A Virus
Viruses
Viral Genes
Point Mutation
Complementary DNA
RNA

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

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Identification and characterization of mutations in the high growth vaccine strain of influenza virus. / Lee, K. H.; Youn, J. W.; Kim, H. J.; Seong, Baik Lin.

In: Archives of Virology, Vol. 146, No. 2, 12.04.2001, p. 369-377.

Research output: Contribution to journalArticle

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