Identification and diagnostic utility of serologic reactive antigens from Mycobacterium tuberculosis sonic extracts

Yu Mi Kwon, Ki Hwan Jung, Go Eun Choi, A. Rum Shin, Byung Su Lee, Choul Jae Won, Woo Sik Kim, Sung Jae Shin, Jeong Kyu Park, Chulhun L. Chang, Hwa Jung Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

It is important to identify and to test serologically active antigens, so as to devise a cocktail of the best antigens or peptides. We searched for antigens that have serodiagnostic utility using two-dimensional fractionation of sonic extracts from Mycobacterium tuberculosis and probing with pools of sera from healthy subjects and patients with tuberculosis (TB). Reactive protein spots with patient sera were identified by tandem mass spectrometry. Three proteins, Rv0652, Rv2626c, and Rv3418c, which have not previously been described as serologic targets, were identified. Rv0652 protein among them was expressed in Escherichia coli and serum IgG antibodies against this antigen were measured in 150 patients with pulmonary TB and in 115 healthy subjects. The sensitivity and specificity were 39% and 92%, respectively. These results suggest that a newly identified protein, Rv0652 may be a valuable candidate to be included in a cocktail test kit for TB diagnosis.

Original languageEnglish
Pages (from-to)329-336
Number of pages8
JournalJournal of Bacteriology and Virology
Volume39
Issue number4
DOIs
Publication statusPublished - 2009

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Fingerprint Dive into the research topics of 'Identification and diagnostic utility of serologic reactive antigens from Mycobacterium tuberculosis sonic extracts'. Together they form a unique fingerprint.

  • Cite this

    Kwon, Y. M., Jung, K. H., Choi, G. E., Shin, A. R., Lee, B. S., Won, C. J., Kim, W. S., Shin, S. J., Park, J. K., Chang, C. L., & Kim, H. J. (2009). Identification and diagnostic utility of serologic reactive antigens from Mycobacterium tuberculosis sonic extracts. Journal of Bacteriology and Virology, 39(4), 329-336. https://doi.org/10.4167/jbv.2009.39.4.329