Identification and Purification of IgE-reactive Proteins in German Cockroach Extract

Tai Soon Yong, Jong Seok Lee, Jongweon Lee, Soon Jung Park, Soung Hoo Jeon, Han Il Ree, Jung Won Park

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cockroaches have been implicated as a cause of respiratory allergy in urban areas world-wide. IgE-reactive German cockroach proteins were identified with molecular weights (MWs) of 90, 66, 50, 43 and 36 KD by immunoblot analysis in both immune BALB/c mice and sensitized humans. Prominent IgE-reactive proteins were purified using FPLC by ion-exchange chromatography, gel filtration and hydrophobic chromatography. The N-terminal amino acid sequence of a purified protein with a MW of 66 KD on SDS-PAGE was Val-Thr-Leu-Lys-Lys(Val)-Met-Ile-Lys-Thr-Phe-Tyr. No homologous protein was found through a search of GenBank that indicated a novel IgE-reactive protein in German cockroach extract. Another purified protein with a MW of 36 KD reacted strongly with a monoclonal antibody against Bla g 2.

Original languageEnglish
Pages (from-to)283-289
Number of pages7
JournalYonsei medical journal
Volume40
Issue number3
DOIs
Publication statusPublished - 1999 Jun

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All Science Journal Classification (ASJC) codes

  • Medicine(all)

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