Identification and use of zinc finger transcription factors that increase production of recombinant proteins in yeast and mammalian cells

Kyung Soon Park, Wongi Seol, Hyo Young Yang, Seong Il Lee, Keun Kim Sung, Jun Kwon Ryuk, Eui Joong Kim, Young Hoon Roh, Lin Seong Baik, Jin Soo Kim

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Randomized ZFP-TF libraries could induce a specific phenotype without detailed knowledge about the phenotype of interest because, theoretically, the libraries could modulate any gene in the target organism. We have developed a novel method for enhancing the efficiency of recombinant protein production in mammalian and microbial cells using combinatorial libraries of zinc finger protein transcription factors. To this end, we constructed tens of thousands of zinc finger proteins (ZFPs) with distinct DNA-binding specificities and fused these ZFPs to either a transcriptional activation or repression domain to make transcriptional activators or repressors, respectively. Expression vectors that encode these artificial transcription factors were delivered into Saccharomyces cerevisiae or HEK 293 cells along with reporter plasmids that code for human growth hormone (hGH) or SEAP (secreted alkaline phosphatase) (for yeast or HEK, respectively). Expression of the reporter genes was driven by either the cytomegalovirus (CMV) or SV40 virus promoters. After transfection, we screened the cells for increased synthesis of the reporter proteins. From these cells, we then isolated several ZFP-transcription factors (ZFP-TFs) that significantly increased hGH or SEAP synthesis and subjected these regulatory proteins to further characterization. Our results show that randomized ZFP-TF libraries are useful tools for improving the yield of heterologous recombinant protein both in yeast and mammalian cells.

Original languageEnglish
Pages (from-to)664-670
Number of pages7
JournalBiotechnology Progress
Volume21
Issue number3
DOIs
Publication statusPublished - 2005 May 1

Fingerprint

Zinc Fingers
Recombinant Proteins
Transcription Factors
Yeasts
Proteins
Libraries
Human Growth Hormone
Alkaline Phosphatase
Phenotype
Simian virus 40
HEK293 Cells
Cytomegalovirus
Reporter Genes
Transcriptional Activation
Transfection
Saccharomyces cerevisiae
Plasmids
DNA
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology

Cite this

Park, Kyung Soon ; Seol, Wongi ; Yang, Hyo Young ; Lee, Seong Il ; Sung, Keun Kim ; Ryuk, Jun Kwon ; Kim, Eui Joong ; Roh, Young Hoon ; Baik, Lin Seong ; Kim, Jin Soo. / Identification and use of zinc finger transcription factors that increase production of recombinant proteins in yeast and mammalian cells. In: Biotechnology Progress. 2005 ; Vol. 21, No. 3. pp. 664-670.
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Identification and use of zinc finger transcription factors that increase production of recombinant proteins in yeast and mammalian cells. / Park, Kyung Soon; Seol, Wongi; Yang, Hyo Young; Lee, Seong Il; Sung, Keun Kim; Ryuk, Jun Kwon; Kim, Eui Joong; Roh, Young Hoon; Baik, Lin Seong; Kim, Jin Soo.

In: Biotechnology Progress, Vol. 21, No. 3, 01.05.2005, p. 664-670.

Research output: Contribution to journalArticle

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