Identification and validation of calmodulin as a binding protein of an anti-proliferative small molecule 3,4-dihydroisoquinolinium salt

Hye Jin Jung, Joong Sup Shim, Jungchan Park, Hyun Joon Ha, Jung Ho Kim, Joong Gon Kim, Nam Doo Kim, Jeong Hyeok Yoon, Ho Jeong Kwon

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

IHY-153 (2-(2,5-difluorobenzyl)-3,4-dihydro-5-(10-hydroxydecyl)-6- methoxy-1-undecylisoquinolinium bromide) was recently discovered as a small molecule that potently inhibits proliferation of tumor cells by inducing cell-cycle arrest at G0-G1 phase. To investigate the basis of anti-proliferative activity of IHY-153, cellular binding proteins of biotinyl-IHY-153 were screened using T7 phage displayed human cDNA libraries. Calmodulin-expressing phage specifically bound to immobilized IHY-153 in a Ca2+-dependent manner. The interaction between IHY-153 and Ca2+//CaM was validated through phage competition binding assays, surface plasmon resonance analysis, and molecular modeling. IHY-153 induced sustained phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 and subsequently increased p21WAF1 expression in colon cancer cells. These results demonstrate that IHY-153, a novel small molecule, targets Ca2+/CaM and indicate that this compound functions as an anti-proliferative agent by influencing Ca2+/CaM- dependent signal transduction.

Original languageEnglish
Pages (from-to)423-432
Number of pages10
JournalProteomics - Clinical Applications
Volume3
Issue number4
DOIs
Publication statusPublished - 2009 May 14

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

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