Identification of a cell cycle-dependent duplicating complex that assembles basal bodies de novo in Naegleria

Jung Ha Lee, Seungmin Kang, Yong Seok Choi, Hong Kyung Kim, Chang Yeol Yeo, Yangsin Lee, Jürgen Roth, Joo Hun Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

During the differentiation of the amoeba Naegleria pringsheimi into a flagellate, a transient complex containing γ-tubulin, pericentrin-like protein, and myosin II (GPM complex) is formed, and subsequently a pair of basal bodies is assembled from the complex. It is not understood, however, how a single GPM is formed nor how the capability to form this complex is acquired by individual cells. We hypothesized that the GPM is formed from a precursor complex and developed an antibody that recognizes Naegleria (Ng)-transacylase, a component of the precursor complex. Immunostaining of differentiating cells showed that Ng-transacylase is concentrated at a site in the amoeba and that γ-tubulin is transiently co-concentrated at the site, suggesting that the GPM is formed from a precursor, GPMp, which contains Ng-transacylase and is already present in the amoeba. Immunostaining of growing N. pringsheimi with Ng-transacylase antibody revealed the presence of one GPMp in interphase cells, but two GPMps in mitotic cells, suggesting that N. pringsheimi maintains one GPMp per cell by duplicating and segregating the complex according to its cell cycle. Our results demonstrate the existence of a cell cycle-dependent duplicating complex that provides a site for the de novo assembly of the next generation of basal bodies.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalProtist
Volume166
Issue number1
DOIs
Publication statusPublished - 2015 Feb 1

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Naegleria
Basal Bodies
Cell Cycle
Amoeba
Tubulin
Myosin Type II
Antibodies
Interphase
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology

Cite this

Lee, Jung Ha ; Kang, Seungmin ; Choi, Yong Seok ; Kim, Hong Kyung ; Yeo, Chang Yeol ; Lee, Yangsin ; Roth, Jürgen ; Lee, Joo Hun. / Identification of a cell cycle-dependent duplicating complex that assembles basal bodies de novo in Naegleria. In: Protist. 2015 ; Vol. 166, No. 1. pp. 1-13.
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Identification of a cell cycle-dependent duplicating complex that assembles basal bodies de novo in Naegleria. / Lee, Jung Ha; Kang, Seungmin; Choi, Yong Seok; Kim, Hong Kyung; Yeo, Chang Yeol; Lee, Yangsin; Roth, Jürgen; Lee, Joo Hun.

In: Protist, Vol. 166, No. 1, 01.02.2015, p. 1-13.

Research output: Contribution to journalArticle

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AU - Lee, Jung Ha

AU - Kang, Seungmin

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AU - Lee, Yangsin

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