Identification of a novel Rb-regulated gene associated with the cell cycle

Young Hoon Sung, Hye Jin Kim, Han Woong Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The retinoblastoma (Rb) gene is one of the most important genes in cell cycle regulation and tumorigenesis. Homozygosity for a germ-line Rb mutation results in embryonic lethality and evokes developmental defects associated with inappropriate S-phase entry and high levels of apoptosis. Although Rb has been extensively studied, more target genes need to be identified and characterized to unravel the precise mechanism of Rb function. In order to identify Rb-regulated genes, we analyzed the gene expression proffle of Rb-deficient mouse embryo fibroblasts (MEFs), and identified an unknown gene, RbEST47, that is transcriptionally upregulated in Rb-deficient MEFs. This gene is conserved from fruitfly to human. It is expressed in brain, lung, kidney, and testis, and is located on mouse chromosome 2. This region is syntenic to human chromosome 9q34.3, which frequently exhibits loss of heterozygosity in neoplastic diseases. RbEST47 was considerably down-regulated in immortalized cells, and showed cell cycle-dependent expression, suggesting important roles in S and/or G2.

Original languageEnglish
Pages (from-to)409-415
Number of pages7
JournalMolecules and cells
Volume24
Issue number3
Publication statusPublished - 2007 Dec 31

Fingerprint

Retinoblastoma Genes
Retinoblastoma
Cell Cycle
Embryonic Structures
Fibroblasts
Genes
cdc Genes
Chromosomes, Human, Pair 2
Germ-Line Mutation
Loss of Heterozygosity
Human Chromosomes
S Phase
Testis
Carcinogenesis
Apoptosis
Kidney
Gene Expression
Lung
Brain

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Sung, Young Hoon ; Kim, Hye Jin ; Lee, Han Woong. / Identification of a novel Rb-regulated gene associated with the cell cycle. In: Molecules and cells. 2007 ; Vol. 24, No. 3. pp. 409-415.
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Identification of a novel Rb-regulated gene associated with the cell cycle. / Sung, Young Hoon; Kim, Hye Jin; Lee, Han Woong.

In: Molecules and cells, Vol. 24, No. 3, 31.12.2007, p. 409-415.

Research output: Contribution to journalArticle

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AB - The retinoblastoma (Rb) gene is one of the most important genes in cell cycle regulation and tumorigenesis. Homozygosity for a germ-line Rb mutation results in embryonic lethality and evokes developmental defects associated with inappropriate S-phase entry and high levels of apoptosis. Although Rb has been extensively studied, more target genes need to be identified and characterized to unravel the precise mechanism of Rb function. In order to identify Rb-regulated genes, we analyzed the gene expression proffle of Rb-deficient mouse embryo fibroblasts (MEFs), and identified an unknown gene, RbEST47, that is transcriptionally upregulated in Rb-deficient MEFs. This gene is conserved from fruitfly to human. It is expressed in brain, lung, kidney, and testis, and is located on mouse chromosome 2. This region is syntenic to human chromosome 9q34.3, which frequently exhibits loss of heterozygosity in neoplastic diseases. RbEST47 was considerably down-regulated in immortalized cells, and showed cell cycle-dependent expression, suggesting important roles in S and/or G2.

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