Identification of a ribosomal frameshift in Leishmania RNA virus 1-4

Sang Eun Lee, Jae Myoung Suh, Scott Scheffter, Jean L. Patterson, In Kwon Chung

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Double-stranded Leishmania RNA virus 1-4 (LRV 1-4) has at least four open Pending frames (ORFs). The two small ORFs located near its 5' terminus, ORF1 and ORFx, could encode 34- and 60-amino acid polypeptides, respectively. ORF2 encodes an 82-kDa major capsid protein, and ORF3 encodes a 98-kDa polypeptide which contains the consensus sequence for RNA-dependent RNA polymerases of plus-strand and double-stranded RNA viruses. The complete sequence of LRV 1-4 shows that ORF2 and ORF3 overlap by 71 nucleotides, and that ORF3 lacks a potential translation initiation site, suggesting that the viral polymerase may be synthesized as a 180-kDa fusion protein with the virus capsid. In this report, we present evidence for the synthesis of a fusion protein through a ribosomal frameshift. In vitro-translation experiments and immunostudies involving antiserum against the viral capsid protein demonstrated that the overlapping 71 nucleotides of ORF2 and ORF3 are contained in a region which promotes translational frameshifting. Computer analysis of the putative frameshift region revealed a potential pseudoknot structure located within the overlapping 71 nucleotide sequence.

Original languageEnglish
Pages (from-to)22-25
Number of pages4
JournalJournal of Biochemistry
Volume120
Issue number1
DOIs
Publication statusPublished - 1996 Jan 1

Fingerprint

Ribosomal Frameshifting
Leishmania
RNA Viruses
Viruses
Double-Stranded RNA
Capsid Proteins
RNA
Open Reading Frames
Nucleotides
Viral Fusion Proteins
RNA Replicase
Peptides
Fusion reactions
Capsid
Consensus Sequence
Viral Proteins
Immune Sera
Amino Acids
Proteins
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Lee, Sang Eun ; Suh, Jae Myoung ; Scheffter, Scott ; Patterson, Jean L. ; Chung, In Kwon. / Identification of a ribosomal frameshift in Leishmania RNA virus 1-4. In: Journal of Biochemistry. 1996 ; Vol. 120, No. 1. pp. 22-25.
@article{be3ef9da0f5b490eaa319035c638b94b,
title = "Identification of a ribosomal frameshift in Leishmania RNA virus 1-4",
abstract = "Double-stranded Leishmania RNA virus 1-4 (LRV 1-4) has at least four open Pending frames (ORFs). The two small ORFs located near its 5' terminus, ORF1 and ORFx, could encode 34- and 60-amino acid polypeptides, respectively. ORF2 encodes an 82-kDa major capsid protein, and ORF3 encodes a 98-kDa polypeptide which contains the consensus sequence for RNA-dependent RNA polymerases of plus-strand and double-stranded RNA viruses. The complete sequence of LRV 1-4 shows that ORF2 and ORF3 overlap by 71 nucleotides, and that ORF3 lacks a potential translation initiation site, suggesting that the viral polymerase may be synthesized as a 180-kDa fusion protein with the virus capsid. In this report, we present evidence for the synthesis of a fusion protein through a ribosomal frameshift. In vitro-translation experiments and immunostudies involving antiserum against the viral capsid protein demonstrated that the overlapping 71 nucleotides of ORF2 and ORF3 are contained in a region which promotes translational frameshifting. Computer analysis of the putative frameshift region revealed a potential pseudoknot structure located within the overlapping 71 nucleotide sequence.",
author = "Lee, {Sang Eun} and Suh, {Jae Myoung} and Scott Scheffter and Patterson, {Jean L.} and Chung, {In Kwon}",
year = "1996",
month = "1",
day = "1",
doi = "10.1093/oxfordjournals.jbchem.a021387",
language = "English",
volume = "120",
pages = "22--25",
journal = "Journal of Biochemistry",
issn = "0021-924X",
publisher = "Oxford University Press",
number = "1",

}

Identification of a ribosomal frameshift in Leishmania RNA virus 1-4. / Lee, Sang Eun; Suh, Jae Myoung; Scheffter, Scott; Patterson, Jean L.; Chung, In Kwon.

In: Journal of Biochemistry, Vol. 120, No. 1, 01.01.1996, p. 22-25.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification of a ribosomal frameshift in Leishmania RNA virus 1-4

AU - Lee, Sang Eun

AU - Suh, Jae Myoung

AU - Scheffter, Scott

AU - Patterson, Jean L.

AU - Chung, In Kwon

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Double-stranded Leishmania RNA virus 1-4 (LRV 1-4) has at least four open Pending frames (ORFs). The two small ORFs located near its 5' terminus, ORF1 and ORFx, could encode 34- and 60-amino acid polypeptides, respectively. ORF2 encodes an 82-kDa major capsid protein, and ORF3 encodes a 98-kDa polypeptide which contains the consensus sequence for RNA-dependent RNA polymerases of plus-strand and double-stranded RNA viruses. The complete sequence of LRV 1-4 shows that ORF2 and ORF3 overlap by 71 nucleotides, and that ORF3 lacks a potential translation initiation site, suggesting that the viral polymerase may be synthesized as a 180-kDa fusion protein with the virus capsid. In this report, we present evidence for the synthesis of a fusion protein through a ribosomal frameshift. In vitro-translation experiments and immunostudies involving antiserum against the viral capsid protein demonstrated that the overlapping 71 nucleotides of ORF2 and ORF3 are contained in a region which promotes translational frameshifting. Computer analysis of the putative frameshift region revealed a potential pseudoknot structure located within the overlapping 71 nucleotide sequence.

AB - Double-stranded Leishmania RNA virus 1-4 (LRV 1-4) has at least four open Pending frames (ORFs). The two small ORFs located near its 5' terminus, ORF1 and ORFx, could encode 34- and 60-amino acid polypeptides, respectively. ORF2 encodes an 82-kDa major capsid protein, and ORF3 encodes a 98-kDa polypeptide which contains the consensus sequence for RNA-dependent RNA polymerases of plus-strand and double-stranded RNA viruses. The complete sequence of LRV 1-4 shows that ORF2 and ORF3 overlap by 71 nucleotides, and that ORF3 lacks a potential translation initiation site, suggesting that the viral polymerase may be synthesized as a 180-kDa fusion protein with the virus capsid. In this report, we present evidence for the synthesis of a fusion protein through a ribosomal frameshift. In vitro-translation experiments and immunostudies involving antiserum against the viral capsid protein demonstrated that the overlapping 71 nucleotides of ORF2 and ORF3 are contained in a region which promotes translational frameshifting. Computer analysis of the putative frameshift region revealed a potential pseudoknot structure located within the overlapping 71 nucleotide sequence.

UR - http://www.scopus.com/inward/record.url?scp=0029737638&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029737638&partnerID=8YFLogxK

U2 - 10.1093/oxfordjournals.jbchem.a021387

DO - 10.1093/oxfordjournals.jbchem.a021387

M3 - Article

C2 - 8864838

AN - SCOPUS:0029737638

VL - 120

SP - 22

EP - 25

JO - Journal of Biochemistry

JF - Journal of Biochemistry

SN - 0021-924X

IS - 1

ER -