Identification of a Short Viral Transcript in Leishmania RNA Virus-infected Cells

In Kwon Chung, Timothy C. Armstrong, Jean L. Patterson

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Certain strains of Leishmania guyanensis carry persistently infecting double-stranded RNA viruses. The viral polymerase has been shown to have both transcriptase and replicase activity. To date, only full-length RNA transcription of minus and plus strands have been reported. This report describes the synthesis of a 320 nt transcript which is complementary to the 3′ end of the minus strand RNA. This plus-stranded short transcript was first detected in an in vitro polymerase assay. It was also detected in virus-infected Leishmania cells by a reverse transcription-polymerase chain reaction assay and by Northern analysis of infected Leishmania cell RNA.

Original languageEnglish
Article number71066
Pages (from-to)552-556
Number of pages5
JournalVirology
Volume198
Issue number2
DOIs
Publication statusPublished - 1994 Feb 1

All Science Journal Classification (ASJC) codes

  • Virology

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