Identification of CD23 as a functional receptor for the proinflammatory cytokine AIMP1/p43

Hyuk Sang Kwon, Min Chul Park, Dae Gyu Kim, Kiwon Cho, Young Woo Park, Jung Min Han, Sunghoon Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Aminoacyl-tRNA-synthetase-interacting multifunctional protein 1 (AIMP1/p43) can be secreted to trigger proinflammatory molecules while it is predominantly bound to a cytoplasmic macromolecular protein complex that contains several different aminoacyl-tRNA synthetases. Although its activities as a secreted signaling factor have been well characterized, the functional receptor for its proinflammatory activity has not yet identified. In this study, we have identified the receptor molecule for AIMP1 that mediates the secretion of TNF-α from THP-1 monocytic cells and primary human peripheral blood mononuclear cells (PBMCs). In a screen of 499 soluble receptors we identified CD23, a known low-affinity receptor for IgE, as a high affinity binding partner of AIMP1.We found that downregulation of CD23 attenuated AIMP1-induced TNF-α secretion and AIMP1 binding to THP-1 and PBMCs. We also observed that in THP-1 and PBMCs, AIMP1-induced TNF-α secretion, mediated by CD23, involved activation of ERK1/2. Interestingly, endothelial monocyte activating polypeptide II (EMAP II), the Cterminal fragment of AIMP1 that is also known to work as a proinflammatory cytokine, was incapable of binding to CD23 and of activating ERK1/2. Therefore, identification of CD23 not only explains the inflammatory function of AIMP1 but also provides the first evidence by which the mode of action of AIMP1 can be distinguished from that of its C-terminal domain, EMAP II.

Original languageEnglish
Pages (from-to)4620-4629
Number of pages10
JournalJournal of cell science
Volume125
Issue number19
DOIs
Publication statusPublished - 2012 Dec 1

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Cytokine Receptors
Amino Acyl-tRNA Synthetases
Blood Cells
IgE Receptors
Multiprotein Complexes
Down-Regulation
Cytokines
Proteins
small inducible cytokine subfamily E, member 1

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Kwon, Hyuk Sang ; Park, Min Chul ; Kim, Dae Gyu ; Cho, Kiwon ; Park, Young Woo ; Han, Jung Min ; Kim, Sunghoon. / Identification of CD23 as a functional receptor for the proinflammatory cytokine AIMP1/p43. In: Journal of cell science. 2012 ; Vol. 125, No. 19. pp. 4620-4629.
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Identification of CD23 as a functional receptor for the proinflammatory cytokine AIMP1/p43. / Kwon, Hyuk Sang; Park, Min Chul; Kim, Dae Gyu; Cho, Kiwon; Park, Young Woo; Han, Jung Min; Kim, Sunghoon.

In: Journal of cell science, Vol. 125, No. 19, 01.12.2012, p. 4620-4629.

Research output: Contribution to journalArticle

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AU - Kwon, Hyuk Sang

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