Identification of cdc25 gene in pinewood nematode, Bursaphelenchus xylophilus, and its function in reproduction

Ye Na Choi, Bong Kyeong Oh, Ichiro Kawasaki, Wan Suk Oh, Yi Lee, Young Ki Paik, Yhong Hee Shim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The cdc25 gene, which is highly conserved in many eukaryotes, encodes a phosphatase that plays essential roles in cell cycle regulation. We identified a cdc25 ortholog in the pinewood nematode, Bursaphelenchus xylophilus. The B. xylophilus ortholog (Bx-cdc25) was found to be highly similar to Caenorhabditis elegans cdc-25.2 in sequence as well as in gene structure, both having long intron 1. The Bx-cdc25 gene was determined to be composed of seven exons and six introns in a 2,580 bp region, and was shown to encode 360 amino acids of a protein containing a highly-conserved phosphatase domain. Bx-cdc25 mRNA was hardly detectable throughout the juvenile stages but was highly expressed in eggs and in both female and male adults. Functional conservation during germline development between C. elegans cdc25 and Bx-cdc25 was revealed by Bx-cdc25 RNA interference in C. elegans.

Original languageEnglish
Pages (from-to)195-201
Number of pages7
JournalMolecules and cells
Volume29
Issue number2
DOIs
Publication statusPublished - 2010 Feb 1

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Caenorhabditis elegans
Reproduction
Phosphoric Monoester Hydrolases
Introns
Genes
RNA Interference
Eukaryota
Eggs
Exons
Cell Cycle
Amino Acids
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Choi, Ye Na ; Oh, Bong Kyeong ; Kawasaki, Ichiro ; Oh, Wan Suk ; Lee, Yi ; Paik, Young Ki ; Shim, Yhong Hee. / Identification of cdc25 gene in pinewood nematode, Bursaphelenchus xylophilus, and its function in reproduction. In: Molecules and cells. 2010 ; Vol. 29, No. 2. pp. 195-201.
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Identification of cdc25 gene in pinewood nematode, Bursaphelenchus xylophilus, and its function in reproduction. / Choi, Ye Na; Oh, Bong Kyeong; Kawasaki, Ichiro; Oh, Wan Suk; Lee, Yi; Paik, Young Ki; Shim, Yhong Hee.

In: Molecules and cells, Vol. 29, No. 2, 01.02.2010, p. 195-201.

Research output: Contribution to journalArticle

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