Identification of Chironomus kiiensis allergens, a dominant species of non-biting midges in Korea.

Taisoon Yong, J. S. Lee, I. Y. Lee, S. J. Park, G. M. Park, H. I. Ree, Jungwon Park, C. S. Hong, H. S. Park

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Non-biting midges are known to contain potent inhalant allergens. IgE antibody responses to the crude extract of Chironomus kiiensis adults, a dominant chironomid species in Korea, were examined. With the IgE-ELISA or passive cutaneous anaphylaxis reactions, increased levels of chironomid-specific IgE were detected in the skin test positive human sera, or immunized BALB/c mouse sera with the crude extract adsorbed to alum. IgE-immunoblot analysis showed major IgE-reacting protein band patterns, which reacted with more than 50% of the skin test positive human sera, at 110, 80, 73, 46, 40, 37, 34, and 31 kDa. The reactive band patterns were largely similar between skin test positive humans and immune BALB/c mice. However, the bands of 55, 31, 27, 26, 24, and 23 kDa were found only in sensitized humans, but not in immunized mice.

Original languageEnglish
Pages (from-to)171-179
Number of pages9
JournalThe Korean journal of parasitology
Volume37
Issue number3
DOIs
Publication statusPublished - 1999 Jan 1

Fingerprint

Chironomidae
Korea
Allergens
Immunoglobulin E
Skin Tests
Complex Mixtures
Serum
Passive Cutaneous Anaphylaxis
Antibody Formation
Enzyme-Linked Immunosorbent Assay
Proteins

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Yong, Taisoon ; Lee, J. S. ; Lee, I. Y. ; Park, S. J. ; Park, G. M. ; Ree, H. I. ; Park, Jungwon ; Hong, C. S. ; Park, H. S. / Identification of Chironomus kiiensis allergens, a dominant species of non-biting midges in Korea. In: The Korean journal of parasitology. 1999 ; Vol. 37, No. 3. pp. 171-179.
@article{d8b5c5ad72714802b596bf14a4f51812,
title = "Identification of Chironomus kiiensis allergens, a dominant species of non-biting midges in Korea.",
abstract = "Non-biting midges are known to contain potent inhalant allergens. IgE antibody responses to the crude extract of Chironomus kiiensis adults, a dominant chironomid species in Korea, were examined. With the IgE-ELISA or passive cutaneous anaphylaxis reactions, increased levels of chironomid-specific IgE were detected in the skin test positive human sera, or immunized BALB/c mouse sera with the crude extract adsorbed to alum. IgE-immunoblot analysis showed major IgE-reacting protein band patterns, which reacted with more than 50{\%} of the skin test positive human sera, at 110, 80, 73, 46, 40, 37, 34, and 31 kDa. The reactive band patterns were largely similar between skin test positive humans and immune BALB/c mice. However, the bands of 55, 31, 27, 26, 24, and 23 kDa were found only in sensitized humans, but not in immunized mice.",
author = "Taisoon Yong and Lee, {J. S.} and Lee, {I. Y.} and Park, {S. J.} and Park, {G. M.} and Ree, {H. I.} and Jungwon Park and Hong, {C. S.} and Park, {H. S.}",
year = "1999",
month = "1",
day = "1",
doi = "10.3347/kjp.1999.37.3.171",
language = "English",
volume = "37",
pages = "171--179",
journal = "Korean Journal of Parasitology",
issn = "0023-4001",
publisher = "Korean Journal of Parasitology",
number = "3",

}

Identification of Chironomus kiiensis allergens, a dominant species of non-biting midges in Korea. / Yong, Taisoon; Lee, J. S.; Lee, I. Y.; Park, S. J.; Park, G. M.; Ree, H. I.; Park, Jungwon; Hong, C. S.; Park, H. S.

In: The Korean journal of parasitology, Vol. 37, No. 3, 01.01.1999, p. 171-179.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification of Chironomus kiiensis allergens, a dominant species of non-biting midges in Korea.

AU - Yong, Taisoon

AU - Lee, J. S.

AU - Lee, I. Y.

AU - Park, S. J.

AU - Park, G. M.

AU - Ree, H. I.

AU - Park, Jungwon

AU - Hong, C. S.

AU - Park, H. S.

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Non-biting midges are known to contain potent inhalant allergens. IgE antibody responses to the crude extract of Chironomus kiiensis adults, a dominant chironomid species in Korea, were examined. With the IgE-ELISA or passive cutaneous anaphylaxis reactions, increased levels of chironomid-specific IgE were detected in the skin test positive human sera, or immunized BALB/c mouse sera with the crude extract adsorbed to alum. IgE-immunoblot analysis showed major IgE-reacting protein band patterns, which reacted with more than 50% of the skin test positive human sera, at 110, 80, 73, 46, 40, 37, 34, and 31 kDa. The reactive band patterns were largely similar between skin test positive humans and immune BALB/c mice. However, the bands of 55, 31, 27, 26, 24, and 23 kDa were found only in sensitized humans, but not in immunized mice.

AB - Non-biting midges are known to contain potent inhalant allergens. IgE antibody responses to the crude extract of Chironomus kiiensis adults, a dominant chironomid species in Korea, were examined. With the IgE-ELISA or passive cutaneous anaphylaxis reactions, increased levels of chironomid-specific IgE were detected in the skin test positive human sera, or immunized BALB/c mouse sera with the crude extract adsorbed to alum. IgE-immunoblot analysis showed major IgE-reacting protein band patterns, which reacted with more than 50% of the skin test positive human sera, at 110, 80, 73, 46, 40, 37, 34, and 31 kDa. The reactive band patterns were largely similar between skin test positive humans and immune BALB/c mice. However, the bands of 55, 31, 27, 26, 24, and 23 kDa were found only in sensitized humans, but not in immunized mice.

UR - http://www.scopus.com/inward/record.url?scp=0033192987&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033192987&partnerID=8YFLogxK

U2 - 10.3347/kjp.1999.37.3.171

DO - 10.3347/kjp.1999.37.3.171

M3 - Article

C2 - 10507225

AN - SCOPUS:0033192987

VL - 37

SP - 171

EP - 179

JO - Korean Journal of Parasitology

JF - Korean Journal of Parasitology

SN - 0023-4001

IS - 3

ER -