TY - JOUR
T1 - Identification of differentially expressed cDNAs in Acanthamoeba culbertsoni after mouse brain passage.
AU - Han, Kyu Lee
AU - Lee, Jongweon
AU - Kim, Don Soo
AU - Park, Soon Jung
AU - Im, Kyung il
AU - Yong, Tai Soon
PY - 2006/3
Y1 - 2006/3
N2 - Free-living amoebae of the genus Acanthamoeba are causative agents of granulomatous amebic encephalitis and amebic keratitis. Because the virulence of Acanthamoeba culbertsoni cultured in the laboratory is restored by consecutive brain passages, we examined the genes induced in mouse brain-passaged A. culbertsoni by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR). Enhanced A. culbertsoni virulence was observed during the second mouse brain passage, i.e., infected mouse mortality increased from 5% to 70%. Ten cDNAs induced during mouse brain passage were identified by DDRT-PCR and this was confirmed by northern blot analysis. BlastX searches of these cDNAs indicated the upregulations of genes encoding predictive NADH-dehydrogenase, proteasomal ATPase, and GDP-mannose pyrophosphorylase B, which have previously been reported to be associated with A. culbertsoni virulence factors.
AB - Free-living amoebae of the genus Acanthamoeba are causative agents of granulomatous amebic encephalitis and amebic keratitis. Because the virulence of Acanthamoeba culbertsoni cultured in the laboratory is restored by consecutive brain passages, we examined the genes induced in mouse brain-passaged A. culbertsoni by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR). Enhanced A. culbertsoni virulence was observed during the second mouse brain passage, i.e., infected mouse mortality increased from 5% to 70%. Ten cDNAs induced during mouse brain passage were identified by DDRT-PCR and this was confirmed by northern blot analysis. BlastX searches of these cDNAs indicated the upregulations of genes encoding predictive NADH-dehydrogenase, proteasomal ATPase, and GDP-mannose pyrophosphorylase B, which have previously been reported to be associated with A. culbertsoni virulence factors.
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U2 - 10.3347/kjp.2006.44.1.15
DO - 10.3347/kjp.2006.44.1.15
M3 - Article
C2 - 16514277
AN - SCOPUS:33646841359
SN - 0023-4001
VL - 44
SP - 15
EP - 20
JO - Korean Journal of Parasitology
JF - Korean Journal of Parasitology
IS - 1
ER -
