Identification of EBP50 as a specific biomarker for carcinogens via the analysis of mouse lymphoma cellular proteome

Yoen Jung Lee, In Kwon Choi, Yhun Yhong Sheen, Sue Nie Park, Ho Jeong Kwon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

To identify specific biomarkers generated upon exposure of L5178Y mouse lymphoma cells to carcinogens, 2-DE and MALDI-TOF MS analysis were conducted using the cellular proteome of L5178Y cells that had been treated with the known carcinogens, 1,2-dibromoethane and O-nitrotoluene and the noncarcinogens, emodin and D-mannitol. Eight protein spots that showed a greater than 1.5-fold increase or decrease in intensity following carcinogen treatment compared with treatment with noncarcinogens were selected. Of the identified proteins, we focused on the candidate biomarker ERM-binding phosphoprotein 50(EBP50), the expression of which was specifically increased in response to treatment with the carcinogens. The expression level of EBP50 was determined by western analysis using polyclonal rabbit anti-EBP50 antibody. Further, the expression level of EBP50 was increased in cells treated with seven additional carcinogens, verifying that EBP50 could serve as a specific biomarker for carcinogens.

Original languageEnglish
Pages (from-to)309-316
Number of pages8
JournalMolecules and cells
Volume33
Issue number3
DOIs
Publication statusPublished - 2012 Mar 1

Fingerprint

Phosphoproteins
Proteome
Carcinogens
Lymphoma
Biomarkers
Ethylene Dibromide
Emodin
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Mannitol
Proteins
Rabbits
Antibodies

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Lee, Yoen Jung ; Choi, In Kwon ; Sheen, Yhun Yhong ; Park, Sue Nie ; Kwon, Ho Jeong. / Identification of EBP50 as a specific biomarker for carcinogens via the analysis of mouse lymphoma cellular proteome. In: Molecules and cells. 2012 ; Vol. 33, No. 3. pp. 309-316.
@article{5eb54c6b50764486b85866978f9a6b1c,
title = "Identification of EBP50 as a specific biomarker for carcinogens via the analysis of mouse lymphoma cellular proteome",
abstract = "To identify specific biomarkers generated upon exposure of L5178Y mouse lymphoma cells to carcinogens, 2-DE and MALDI-TOF MS analysis were conducted using the cellular proteome of L5178Y cells that had been treated with the known carcinogens, 1,2-dibromoethane and O-nitrotoluene and the noncarcinogens, emodin and D-mannitol. Eight protein spots that showed a greater than 1.5-fold increase or decrease in intensity following carcinogen treatment compared with treatment with noncarcinogens were selected. Of the identified proteins, we focused on the candidate biomarker ERM-binding phosphoprotein 50(EBP50), the expression of which was specifically increased in response to treatment with the carcinogens. The expression level of EBP50 was determined by western analysis using polyclonal rabbit anti-EBP50 antibody. Further, the expression level of EBP50 was increased in cells treated with seven additional carcinogens, verifying that EBP50 could serve as a specific biomarker for carcinogens.",
author = "Lee, {Yoen Jung} and Choi, {In Kwon} and Sheen, {Yhun Yhong} and Park, {Sue Nie} and Kwon, {Ho Jeong}",
year = "2012",
month = "3",
day = "1",
doi = "10.1007/s10059-012-2280-7",
language = "English",
volume = "33",
pages = "309--316",
journal = "Molecules and Cells",
issn = "1016-8478",
publisher = "Korean Society for Molecular and Cellular Biology",
number = "3",

}

Identification of EBP50 as a specific biomarker for carcinogens via the analysis of mouse lymphoma cellular proteome. / Lee, Yoen Jung; Choi, In Kwon; Sheen, Yhun Yhong; Park, Sue Nie; Kwon, Ho Jeong.

In: Molecules and cells, Vol. 33, No. 3, 01.03.2012, p. 309-316.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification of EBP50 as a specific biomarker for carcinogens via the analysis of mouse lymphoma cellular proteome

AU - Lee, Yoen Jung

AU - Choi, In Kwon

AU - Sheen, Yhun Yhong

AU - Park, Sue Nie

AU - Kwon, Ho Jeong

PY - 2012/3/1

Y1 - 2012/3/1

N2 - To identify specific biomarkers generated upon exposure of L5178Y mouse lymphoma cells to carcinogens, 2-DE and MALDI-TOF MS analysis were conducted using the cellular proteome of L5178Y cells that had been treated with the known carcinogens, 1,2-dibromoethane and O-nitrotoluene and the noncarcinogens, emodin and D-mannitol. Eight protein spots that showed a greater than 1.5-fold increase or decrease in intensity following carcinogen treatment compared with treatment with noncarcinogens were selected. Of the identified proteins, we focused on the candidate biomarker ERM-binding phosphoprotein 50(EBP50), the expression of which was specifically increased in response to treatment with the carcinogens. The expression level of EBP50 was determined by western analysis using polyclonal rabbit anti-EBP50 antibody. Further, the expression level of EBP50 was increased in cells treated with seven additional carcinogens, verifying that EBP50 could serve as a specific biomarker for carcinogens.

AB - To identify specific biomarkers generated upon exposure of L5178Y mouse lymphoma cells to carcinogens, 2-DE and MALDI-TOF MS analysis were conducted using the cellular proteome of L5178Y cells that had been treated with the known carcinogens, 1,2-dibromoethane and O-nitrotoluene and the noncarcinogens, emodin and D-mannitol. Eight protein spots that showed a greater than 1.5-fold increase or decrease in intensity following carcinogen treatment compared with treatment with noncarcinogens were selected. Of the identified proteins, we focused on the candidate biomarker ERM-binding phosphoprotein 50(EBP50), the expression of which was specifically increased in response to treatment with the carcinogens. The expression level of EBP50 was determined by western analysis using polyclonal rabbit anti-EBP50 antibody. Further, the expression level of EBP50 was increased in cells treated with seven additional carcinogens, verifying that EBP50 could serve as a specific biomarker for carcinogens.

UR - http://www.scopus.com/inward/record.url?scp=84863449883&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863449883&partnerID=8YFLogxK

U2 - 10.1007/s10059-012-2280-7

DO - 10.1007/s10059-012-2280-7

M3 - Article

C2 - 22434383

AN - SCOPUS:84863449883

VL - 33

SP - 309

EP - 316

JO - Molecules and Cells

JF - Molecules and Cells

SN - 1016-8478

IS - 3

ER -