To identify specific biomarkers generated upon exposure of L5178Y mouse lymphoma cells to carcinogens, 2-DE and MALDI-TOF MS analysis were conducted using the cellular proteome of L5178Y cells that had been treated with the known carcinogens, 1,2-dibromoethane and O-nitrotoluene and the noncarcinogens, emodin and D-mannitol. Eight protein spots that showed a greater than 1.5-fold increase or decrease in intensity following carcinogen treatment compared with treatment with noncarcinogens were selected. Of the identified proteins, we focused on the candidate biomarker ERM-binding phosphoprotein 50(EBP50), the expression of which was specifically increased in response to treatment with the carcinogens. The expression level of EBP50 was determined by western analysis using polyclonal rabbit anti-EBP50 antibody. Further, the expression level of EBP50 was increased in cells treated with seven additional carcinogens, verifying that EBP50 could serve as a specific biomarker for carcinogens.
Bibliographical noteFunding Information:
This study was partly supported by grants from the Division of Genetic Toxicology National Institute of Toxicological Research, KFDA, the National Research Foundation of Korea funded by the Korean Government (MEST; 2009-0092964, 2010-0017984, F01-2009-000-10183-0), Translational Research Center for Protein Function Control, the Next-Generation BioGreen 21 Program (2011-8-1178), Rural Development Administration, National R&D Program, Ministry of Health & Welfare, and from the Brain Korea 21 Project, Republic of Korea. We thank Drs. Young-ki Paik and Jong Shin Yoo for their valuable comments and Korea Basic Science Institute (KBSI) and Yonsei Proteome Research Center (YPRC) for technical support of proteomic analysis.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology