Identification of Elements Responsible for Maternally-Silenced Imprinted Gene Expression of Upward Curly Leaf1, an F-box Protein Gene that Regulates Curly Leaf in Arabidopsis

Jooyeon Hong, Jaehoon Lee, Cheol Woong Jeong, Janie Sue Brooks, Yeonhee Choi, Jong Seob Lee

Research output: Contribution to journalArticlepeer-review

Abstract

Upward Curly Leaf 1 (UCL1) is an Arabidopsis thaliana E3 ligase that targets the Curly Leaf (CLF) SET-domain polycomb-group (PcG) protein for degradation via the ubiquitin-26S proteasome system. UCL1 is a paternally-expressed imprinted gene in the endosperm. To precisely locate the promoter elements required for UCL1 imprinting pattern, various gene constructs were created in which the imprinting control region (ICR), endosperm-specific expression (ENSE) element, and/or the linker sequence were altered. By fusing these constructs with a GUS reporter gene, GUS expression patterns were monitored after reciprocal crosses with wild-type Columbia-0 allowing the determination of parent-of-origin expression. Analysis of publicly-available data on the UCL1 promoter region facilitated the search for allele-specific DNA and H3K27 methylation patterns. Overall, three promoter elements are required for maternal repression of UCL1; the ICR sequence located from − 2.5 to − 2.4 kb upstream of the translation start site, a differentially methylated region 2 (DMR2) that overlaps the short ATLINE1-1 transposable element in the linker region, and a minimal 271 bp ENSE element. In addition, DNA methylation patterns in the DMR2 contribute to the repression of the maternal UCL1 allele. Our findings would help to understand how parent-of-origin epigenetic patterns are created and maintained in the endosperm.

Original languageEnglish
Pages (from-to)337-346
Number of pages10
JournalJournal of Plant Biology
Volume63
Issue number5
DOIs
Publication statusPublished - 2020 Oct 1

Bibliographical note

Funding Information:
The work was supported by NRF of Korea (2015R1A2A2A01004095) to J.S.L. This work was also supported by NRF of Korea (2020R1A2C2009382) to Y.C. Both J.H. and J.L. were supported by the Stadelmann-Lee Scholarship Fund, Seoul National University.

All Science Journal Classification (ASJC) codes

  • Plant Science

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