Abstract
Yeast two-hybrid assays using the end-binding 1 (EB1) protein of Giardia lamblia as bait led to the isolation of four independent clones encoding γ-giardin, a neurogenic Notch-like protein, and two hypothetical proteins. Interactions between EB1 and the isolated proteins were confirmed by co-immunoprecipitation of in vitro-synthesized candidate proteins and EB1. Localization of these isolated proteins in G. lamblia was examined by immunofluorescence assays, indicating that they were predominantly present in cytoskeletal structures of G. lamblia. These results suggest that EB1 protein functions via interaction with the cytoskeletal components of G. lamblia.
| Original language | English |
|---|---|
| Pages (from-to) | 723-728 |
| Number of pages | 6 |
| Journal | Parasitology Research |
| Volume | 106 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2010 Feb 1 |
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All Science Journal Classification (ASJC) codes
- Parasitology
- Infectious Diseases
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Identification of end-binding 1 (EB1) interacting proteins in Giardia lamblia. / Kang, Kyungpil; Kim, Juri; Yong, Taisoon; Park, Soon Jung.
In: Parasitology Research, Vol. 106, No. 3, 01.02.2010, p. 723-728.Research output: Contribution to journal › Article
TY - JOUR
T1 - Identification of end-binding 1 (EB1) interacting proteins in Giardia lamblia
AU - Kang, Kyungpil
AU - Kim, Juri
AU - Yong, Taisoon
AU - Park, Soon Jung
PY - 2010/2/1
Y1 - 2010/2/1
N2 - Yeast two-hybrid assays using the end-binding 1 (EB1) protein of Giardia lamblia as bait led to the isolation of four independent clones encoding γ-giardin, a neurogenic Notch-like protein, and two hypothetical proteins. Interactions between EB1 and the isolated proteins were confirmed by co-immunoprecipitation of in vitro-synthesized candidate proteins and EB1. Localization of these isolated proteins in G. lamblia was examined by immunofluorescence assays, indicating that they were predominantly present in cytoskeletal structures of G. lamblia. These results suggest that EB1 protein functions via interaction with the cytoskeletal components of G. lamblia.
AB - Yeast two-hybrid assays using the end-binding 1 (EB1) protein of Giardia lamblia as bait led to the isolation of four independent clones encoding γ-giardin, a neurogenic Notch-like protein, and two hypothetical proteins. Interactions between EB1 and the isolated proteins were confirmed by co-immunoprecipitation of in vitro-synthesized candidate proteins and EB1. Localization of these isolated proteins in G. lamblia was examined by immunofluorescence assays, indicating that they were predominantly present in cytoskeletal structures of G. lamblia. These results suggest that EB1 protein functions via interaction with the cytoskeletal components of G. lamblia.
UR - http://www.scopus.com/inward/record.url?scp=77949270727&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77949270727&partnerID=8YFLogxK
U2 - 10.1007/s00436-009-1687-3
DO - 10.1007/s00436-009-1687-3
M3 - Article
C2 - 19953272
AN - SCOPUS:77949270727
VL - 106
SP - 723
EP - 728
JO - Parasitology Research
JF - Parasitology Research
SN - 0932-0113
IS - 3
ER -