Identification of end-binding 1 (EB1) interacting proteins in Giardia lamblia

Kyungpil Kang, Juri Kim, Tai Soon Yong, Soon Jung Park

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5 Citations (Scopus)

Abstract

Yeast two-hybrid assays using the end-binding 1 (EB1) protein of Giardia lamblia as bait led to the isolation of four independent clones encoding γ-giardin, a neurogenic Notch-like protein, and two hypothetical proteins. Interactions between EB1 and the isolated proteins were confirmed by co-immunoprecipitation of in vitro-synthesized candidate proteins and EB1. Localization of these isolated proteins in G. lamblia was examined by immunofluorescence assays, indicating that they were predominantly present in cytoskeletal structures of G. lamblia. These results suggest that EB1 protein functions via interaction with the cytoskeletal components of G. lamblia.

Original languageEnglish
Pages (from-to)723-728
Number of pages6
JournalParasitology Research
Volume106
Issue number3
DOIs
Publication statusPublished - 2010 Feb

Bibliographical note

Funding Information:
Acknowledgments This study was supported by Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Education, Science and Technology (2009-0078120 to S.-J.P), Republic of Korea.

All Science Journal Classification (ASJC) codes

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

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