Identification of end-binding 1 (EB1) interacting proteins in Giardia lamblia

Kyungpil Kang; Juri Kim; Tai Soon Yong; Soon Jung Park

doi:10.1007/s00436-009-1687-3

Identification of end-binding 1 (EB1) interacting proteins in Giardia lamblia

Kyungpil Kang, Juri Kim, Tai Soon Yong, Soon Jung Park

Department of Environmental Medical Biology

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Yeast two-hybrid assays using the end-binding 1 (EB1) protein of Giardia lamblia as bait led to the isolation of four independent clones encoding γ-giardin, a neurogenic Notch-like protein, and two hypothetical proteins. Interactions between EB1 and the isolated proteins were confirmed by co-immunoprecipitation of in vitro-synthesized candidate proteins and EB1. Localization of these isolated proteins in G. lamblia was examined by immunofluorescence assays, indicating that they were predominantly present in cytoskeletal structures of G. lamblia. These results suggest that EB1 protein functions via interaction with the cytoskeletal components of G. lamblia.

Original language	English
Pages (from-to)	723-728
Number of pages	6
Journal	Parasitology Research
Volume	106
Issue number	3
DOIs	https://doi.org/10.1007/s00436-009-1687-3
Publication status	Published - 2010 Feb

Bibliographical note

Funding Information:
Acknowledgments This study was supported by Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Education, Science and Technology (2009-0078120 to S.-J.P), Republic of Korea.

All Science Journal Classification (ASJC) codes

Parasitology
veterinary(all)
Insect Science
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00436-009-1687-3

Cite this

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title = "Identification of end-binding 1 (EB1) interacting proteins in Giardia lamblia",

abstract = "Yeast two-hybrid assays using the end-binding 1 (EB1) protein of Giardia lamblia as bait led to the isolation of four independent clones encoding γ-giardin, a neurogenic Notch-like protein, and two hypothetical proteins. Interactions between EB1 and the isolated proteins were confirmed by co-immunoprecipitation of in vitro-synthesized candidate proteins and EB1. Localization of these isolated proteins in G. lamblia was examined by immunofluorescence assays, indicating that they were predominantly present in cytoskeletal structures of G. lamblia. These results suggest that EB1 protein functions via interaction with the cytoskeletal components of G. lamblia.",

author = "Kyungpil Kang and Juri Kim and Yong, {Tai Soon} and Park, {Soon Jung}",

note = "Funding Information: Acknowledgments This study was supported by Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Education, Science and Technology (2009-0078120 to S.-J.P), Republic of Korea.",

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doi = "10.1007/s00436-009-1687-3",

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AU - Kang, Kyungpil

AU - Kim, Juri

AU - Yong, Tai Soon

AU - Park, Soon Jung

N1 - Funding Information: Acknowledgments This study was supported by Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Education, Science and Technology (2009-0078120 to S.-J.P), Republic of Korea.

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AB - Yeast two-hybrid assays using the end-binding 1 (EB1) protein of Giardia lamblia as bait led to the isolation of four independent clones encoding γ-giardin, a neurogenic Notch-like protein, and two hypothetical proteins. Interactions between EB1 and the isolated proteins were confirmed by co-immunoprecipitation of in vitro-synthesized candidate proteins and EB1. Localization of these isolated proteins in G. lamblia was examined by immunofluorescence assays, indicating that they were predominantly present in cytoskeletal structures of G. lamblia. These results suggest that EB1 protein functions via interaction with the cytoskeletal components of G. lamblia.

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Identification of end-binding 1 (EB1) interacting proteins in Giardia lamblia

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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