Identification of end-binding 1 (EB1) interacting proteins in Giardia lamblia

Kyungpil Kang, Juri Kim, Taisoon Yong, Soon Jung Park

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Yeast two-hybrid assays using the end-binding 1 (EB1) protein of Giardia lamblia as bait led to the isolation of four independent clones encoding γ-giardin, a neurogenic Notch-like protein, and two hypothetical proteins. Interactions between EB1 and the isolated proteins were confirmed by co-immunoprecipitation of in vitro-synthesized candidate proteins and EB1. Localization of these isolated proteins in G. lamblia was examined by immunofluorescence assays, indicating that they were predominantly present in cytoskeletal structures of G. lamblia. These results suggest that EB1 protein functions via interaction with the cytoskeletal components of G. lamblia.

Original languageEnglish
Pages (from-to)723-728
Number of pages6
JournalParasitology Research
Volume106
Issue number3
DOIs
Publication statusPublished - 2010 Feb 1

Fingerprint

Giardia lamblia
Carrier Proteins
Proteins
proteins
Notch Receptors
Two-Hybrid System Techniques
Immunoprecipitation
Protein Binding
Fluorescent Antibody Technique
Clone Cells
two hybrid system techniques
baits
fluorescent antibody technique
clones
assays

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Kang, Kyungpil ; Kim, Juri ; Yong, Taisoon ; Park, Soon Jung. / Identification of end-binding 1 (EB1) interacting proteins in Giardia lamblia. In: Parasitology Research. 2010 ; Vol. 106, No. 3. pp. 723-728.
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Identification of end-binding 1 (EB1) interacting proteins in Giardia lamblia. / Kang, Kyungpil; Kim, Juri; Yong, Taisoon; Park, Soon Jung.

In: Parasitology Research, Vol. 106, No. 3, 01.02.2010, p. 723-728.

Research output: Contribution to journalArticle

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