Identification of follicular dendritic cell membrane proteome by LC/MS/MS

Jong Sik Lee, Yoon Park, Hae Kyung Jeon, Tae H. Lee, Jong Bok Yoon

Research output: Contribution to conferencePaper

Abstract

Proteins present in follicular dendritic cell (FDC) membrane were identified using LC/MS/MS method. Reducing SDS-PAGE was performed on the crude plasma membranes using a 10cm2×1.0mm precast polyacrylamide gel. The LC/MS/MS analysis of each slice generated 100 to 200 peptide sequences which were derived from 20 to 30 unique proteins. A total of 522 unique proteins were identified from the FDC membrane preparation with most of them identified by multiple peptides.

Original languageEnglish
Pages221-222
Number of pages2
Publication statusPublished - 2002 Dec 1
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2002 Jun 22002 Jun 6

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period02/6/202/6/6

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Proteome
Cell membranes
Peptides
Proteins
Dendritic Cells

All Science Journal Classification (ASJC) codes

  • Spectroscopy

Cite this

Lee, J. S., Park, Y., Jeon, H. K., Lee, T. H., & Yoon, J. B. (2002). Identification of follicular dendritic cell membrane proteome by LC/MS/MS. 221-222. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.
Lee, Jong Sik ; Park, Yoon ; Jeon, Hae Kyung ; Lee, Tae H. ; Yoon, Jong Bok. / Identification of follicular dendritic cell membrane proteome by LC/MS/MS. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.2 p.
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Lee, JS, Park, Y, Jeon, HK, Lee, TH & Yoon, JB 2002, 'Identification of follicular dendritic cell membrane proteome by LC/MS/MS', Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 02/6/2 - 02/6/6 pp. 221-222.

Identification of follicular dendritic cell membrane proteome by LC/MS/MS. / Lee, Jong Sik; Park, Yoon; Jeon, Hae Kyung; Lee, Tae H.; Yoon, Jong Bok.

2002. 221-222 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conferencePaper

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Lee JS, Park Y, Jeon HK, Lee TH, Yoon JB. Identification of follicular dendritic cell membrane proteome by LC/MS/MS. 2002. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.