Proteins present in follicular dendritic cell (FDC) membrane were identified using LC/MS/MS method. Reducing SDS-PAGE was performed on the crude plasma membranes using a 10cm2×1.0mm precast polyacrylamide gel. The LC/MS/MS analysis of each slice generated 100 to 200 peptide sequences which were derived from 20 to 30 unique proteins. A total of 522 unique proteins were identified from the FDC membrane preparation with most of them identified by multiple peptides.
|Number of pages||2|
|Publication status||Published - 2002 Dec 1|
|Event||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States|
Duration: 2002 Jun 2 → 2002 Jun 6
|Other||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics|
|Period||02/6/2 → 02/6/6|
All Science Journal Classification (ASJC) codes