Identification of follicular dendritic cell membrane proteome by LC/MS/MS

Jong Sik Lee; Yoon Park; Hae Kyung Jeon; Tae H. Lee; Jong Bok Yoon

Identification of follicular dendritic cell membrane proteome by LC/MS/MS

Jong Sik Lee, Yoon Park, Hae Kyung Jeon, Tae H. Lee, Jong Bok Yoon

Research output: Contribution to conference › Paper › peer-review

Abstract

Proteins present in follicular dendritic cell (FDC) membrane were identified using LC/MS/MS method. Reducing SDS-PAGE was performed on the crude plasma membranes using a 10cm²×1.0mm precast polyacrylamide gel. The LC/MS/MS analysis of each slice generated 100 to 200 peptide sequences which were derived from 20 to 30 unique proteins. A total of 522 unique proteins were identified from the FDC membrane preparation with most of them identified by multiple peptides.

Original language	English
Pages	221-222
Number of pages	2
Publication status	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2002 Jun 2 → 2002 Jun 6

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	02/6/2 → 02/6/6

All Science Journal Classification (ASJC) codes

Spectroscopy

Cite this

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title = "Identification of follicular dendritic cell membrane proteome by LC/MS/MS",

abstract = "Proteins present in follicular dendritic cell (FDC) membrane were identified using LC/MS/MS method. Reducing SDS-PAGE was performed on the crude plasma membranes using a 10cm2×1.0mm precast polyacrylamide gel. The LC/MS/MS analysis of each slice generated 100 to 200 peptide sequences which were derived from 20 to 30 unique proteins. A total of 522 unique proteins were identified from the FDC membrane preparation with most of them identified by multiple peptides.",

author = "Lee, {Jong Sik} and Yoon Park and Jeon, {Hae Kyung} and Lee, {Tae H.} and Yoon, {Jong Bok}",

year = "2002",

language = "English",

pages = "221--222",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - CONF

T1 - Identification of follicular dendritic cell membrane proteome by LC/MS/MS

AU - Lee, Jong Sik

AU - Park, Yoon

AU - Jeon, Hae Kyung

AU - Lee, Tae H.

AU - Yoon, Jong Bok

PY - 2002

Y1 - 2002

N2 - Proteins present in follicular dendritic cell (FDC) membrane were identified using LC/MS/MS method. Reducing SDS-PAGE was performed on the crude plasma membranes using a 10cm2×1.0mm precast polyacrylamide gel. The LC/MS/MS analysis of each slice generated 100 to 200 peptide sequences which were derived from 20 to 30 unique proteins. A total of 522 unique proteins were identified from the FDC membrane preparation with most of them identified by multiple peptides.

AB - Proteins present in follicular dendritic cell (FDC) membrane were identified using LC/MS/MS method. Reducing SDS-PAGE was performed on the crude plasma membranes using a 10cm2×1.0mm precast polyacrylamide gel. The LC/MS/MS analysis of each slice generated 100 to 200 peptide sequences which were derived from 20 to 30 unique proteins. A total of 522 unique proteins were identified from the FDC membrane preparation with most of them identified by multiple peptides.

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UR - http://www.scopus.com/inward/citedby.url?scp=2442705601&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442705601

SP - 221

EP - 222

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Identification of follicular dendritic cell membrane proteome by LC/MS/MS

Abstract

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All Science Journal Classification (ASJC) codes

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