Identification of follicular dendritic cell membrane proteome by LC/MS/MS

Jong Sik Lee, Yoon Park, Hae Kyung Jeon, Tae H. Lee, Jong Bok Yoon

Research output: Contribution to conferencePaperpeer-review

Abstract

Proteins present in follicular dendritic cell (FDC) membrane were identified using LC/MS/MS method. Reducing SDS-PAGE was performed on the crude plasma membranes using a 10cm2×1.0mm precast polyacrylamide gel. The LC/MS/MS analysis of each slice generated 100 to 200 peptide sequences which were derived from 20 to 30 unique proteins. A total of 522 unique proteins were identified from the FDC membrane preparation with most of them identified by multiple peptides.

Original languageEnglish
Pages221-222
Number of pages2
Publication statusPublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2002 Jun 22002 Jun 6

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period02/6/202/6/6

All Science Journal Classification (ASJC) codes

  • Spectroscopy

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