Identification of Matrix Metalloproteinase-1-Suppressive Peptides in Feather Keratin Hydrolysate

Hyeon Su Jin, Kyeongseop Song, Je Hyun Baek, Jae Eun Lee, Da Jeong Kim, Gae Won Nam, Nam Joo Kang, Dong Woo Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Inhibition of matrix metalloproteinases (MMPs), which degrade collagen and elastin in the dermis of normal skin, is a key strategy for anti-skin aging. In this study, we identified five low-molecular-weight (LMW, <1 kDa) MMP-1-suppressive peptides in feather keratin hydrolysate (FKH) obtained by anaerobic digestion with an extremophilic bacterium. FKH was first subjected to ultrafiltration, followed by size-exclusion chromatography and liquid chromatography/electrospray ionization tandem mass spectrometry analysis. Chemically synthesized peptides identical to the sequences identified suppressed MMP expression in human dermal fibroblasts (HDFs). To investigate the impact of the MMP-1-suppressive peptides on the signaling pathway, we performed antibody array phosphorylation profiling of HDFs. The results suggested that the peptide GGFDL regulates ultraviolet-B-induced MMP-1 expression by inhibiting mitogen-activated protein kinases and nuclear factor κB signaling pathways as well as histone modification. Thus, LMW feather keratin peptides could serve as novel bioactive compounds to protect the skin against intrinsic and extrinsic factors.

Original languageEnglish
Pages (from-to)12719-12729
Number of pages11
JournalJournal of Agricultural and Food Chemistry
Volume66
Issue number48
DOIs
Publication statusPublished - 2018 Dec 5

Fingerprint

interstitial collagenase
Feathers
Matrix Metalloproteinase 1
keratin
Keratins
hydrolysates
feathers
peptides
Peptides
skin (animal)
Skin
metalloproteinases
Fibroblasts
Matrix Metalloproteinases
fibroblasts
Histone Code
Skin Aging
elastin
Electrospray ionization
Intrinsic Factor

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Jin, Hyeon Su ; Song, Kyeongseop ; Baek, Je Hyun ; Lee, Jae Eun ; Kim, Da Jeong ; Nam, Gae Won ; Kang, Nam Joo ; Lee, Dong Woo. / Identification of Matrix Metalloproteinase-1-Suppressive Peptides in Feather Keratin Hydrolysate. In: Journal of Agricultural and Food Chemistry. 2018 ; Vol. 66, No. 48. pp. 12719-12729.
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Identification of Matrix Metalloproteinase-1-Suppressive Peptides in Feather Keratin Hydrolysate. / Jin, Hyeon Su; Song, Kyeongseop; Baek, Je Hyun; Lee, Jae Eun; Kim, Da Jeong; Nam, Gae Won; Kang, Nam Joo; Lee, Dong Woo.

In: Journal of Agricultural and Food Chemistry, Vol. 66, No. 48, 05.12.2018, p. 12719-12729.

Research output: Contribution to journalArticle

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AU - Jin, Hyeon Su

AU - Song, Kyeongseop

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AU - Kim, Da Jeong

AU - Nam, Gae Won

AU - Kang, Nam Joo

AU - Lee, Dong Woo

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