Identification of MicroRNAs as Potential Blood-Based Biomarkers for Diagnosis and Therapeutic Monitoring of Active Tuberculosis

Junseong Kim, Heechul Park, Sung Bae Park, Eun Ju Lee, Min A. Je, Eunsol Ahn, Bora Sim, Jiyoung Lee, Hyunwoo Jin, Kyung Eun Lee, Sang Nae Cho, Young Ae Kang, Hyejon Lee, Sunghyun Kim, Jungho Kim

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4 Citations (Scopus)


Early diagnosis increases the treatment success rate for active tuberculosis (ATB) and de-creases mortality. MicroRNAs (miRNAs) have been studied as blood-based markers of several in-fectious diseases. We performed miRNA profiling to identify differentially expressed (DE) miRNAs using whole blood samples from 10 healthy controls (HCs), 15 subjects with latent tuberculosis infection (LTBI), and 12 patients with ATB, and investigated the expression of the top six miRNAs at diagnosis and over the treatment period in addition to performing miRNA-target gene network and gene ontology analyses. miRNA profiling identified 84 DE miRNAs in patients with ATB, including 80 upregulated and four downregulated miRNAs. Receiver operating characteristic curves of the top six miRNAs exhibited excellent distinguishing efficiency with an area under curve (AUC) value > 0.85. Among them, miR-199a-3p and miR-6886-3p can differentiate between ATB and LTBI. Anti-TB treatment restored the levels of miR-199b-3p, miR-199a-3p, miR-16-5p, and miR-374c-5p to HC levels. Furthermore, 108 predicted target genes were related to the regulation of cellular amide me-tabolism, intrinsic apoptotic signaling, translation, transforming growth factor beta receptor signal-ing, and cysteine-type endopeptidase activity. The DE miRNAs identified herein are potential bi-omarkers for diagnosis and therapeutic monitoring in ATB.

Original languageEnglish
Article number369
Issue number2
Publication statusPublished - 2022 Feb

Bibliographical note

Funding Information:
Funding: This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, and Future Planning (NRF-2020R1F1A1052068), and by the Catholic University of Pusan 2021.

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry


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