Identification of Missing Proteins in Human Olfactory Epithelial Tissue by Liquid Chromatography-Tandem Mass Spectrometry

Heeyoun Hwang, Ji Eun Jeong, Hyun Kyoung Lee, Ki Na Yun, Hyun Joo An, Bonghee Lee, Young Ki Paik, Tae Seok Jeong, Gi Taek Yee, Jin Young Kim, Jong Shin Yoo

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7 Citations (Scopus)

Abstract

We performed proteomic analyses of human olfactory epithelial tissue to identify missing proteins using liquid chromatography-tandem mass spectrometry. Using a next-generation proteomic pipeline with a < 1.0% false discovery rate at the peptide and protein levels, we identified 3731 proteins, among which five were missing proteins (P0C7M7, P46721, P59826, Q658L1, and Q8N434). We validated the identified missing proteins using the corresponding synthetic peptides. No olfactory receptor (OR) proteins were detected in olfactory tissue, suggesting that detection of ORs would be very difficult. We also identified 49 and 50 alternative splicing variants mapped at the neXtProt and GENCODE databases, respectively, and 2000 additional single amino acid variants. This data set is available at the ProteomeXchange consortium via PRIDE repository (PXD010025).

Original languageEnglish
Pages (from-to)4320-4324
Number of pages5
JournalJournal of Proteome Research
Volume17
Issue number12
DOIs
Publication statusPublished - 2018 Dec 7

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

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    Hwang, H., Jeong, J. E., Lee, H. K., Yun, K. N., An, H. J., Lee, B., Paik, Y. K., Jeong, T. S., Yee, G. T., Kim, J. Y., & Yoo, J. S. (2018). Identification of Missing Proteins in Human Olfactory Epithelial Tissue by Liquid Chromatography-Tandem Mass Spectrometry. Journal of Proteome Research, 17(12), 4320-4324. https://doi.org/10.1021/acs.jproteome.8b00408