Identification of Missing Proteins in Human Olfactory Epithelial Tissue by Liquid Chromatography-Tandem Mass Spectrometry

Heeyoun Hwang, Ji Eun Jeong, Hyun Kyoung Lee, Ki Na Yun, Hyun Joo An, Bonghee Lee, Young Ki Paik, Tae Seok Jeong, Gi Taek Yee, Jin Young Kim, Jong Shin Yoo

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

We performed proteomic analyses of human olfactory epithelial tissue to identify missing proteins using liquid chromatography-tandem mass spectrometry. Using a next-generation proteomic pipeline with a < 1.0% false discovery rate at the peptide and protein levels, we identified 3731 proteins, among which five were missing proteins (P0C7M7, P46721, P59826, Q658L1, and Q8N434). We validated the identified missing proteins using the corresponding synthetic peptides. No olfactory receptor (OR) proteins were detected in olfactory tissue, suggesting that detection of ORs would be very difficult. We also identified 49 and 50 alternative splicing variants mapped at the neXtProt and GENCODE databases, respectively, and 2000 additional single amino acid variants. This data set is available at the ProteomeXchange consortium via PRIDE repository (PXD010025).

Original languageEnglish
Pages (from-to)4320-4324
Number of pages5
JournalJournal of Proteome Research
Volume17
Issue number12
DOIs
Publication statusPublished - 2018 Dec 7

Bibliographical note

Funding Information:
This research was supported by the National Research Council of Science and Technology (NTM2371511, the Creative Allied Project [CAP]), the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI); it was funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI13C2098), and by the National Research Foundation of Korea Grant funded by the Korean Government (MSIP) (2017, R&D Equipment Engineer Education Program, 2014R1A6A9064166).

Publisher Copyright:
Copyright © 2018 American Chemical Society.

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

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