Identification of Mycobacterium species in FFPE granulomatous lymphadenitis tissue using REBA Myco-ID®

J. Munkhdelger, H. Y. Wang, Y. Choi, P. M. Wairagu, D. Lee, S. Park, S. Kim, B. Y. Jeon, H. Lee, K. H. Park

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

SETTING: Tuberculous lymphadenitis is the most common form of lymphadenopathy; its main histopathological finding is granulomatous inflammation. OBJECTIVE: A reverse blot hybridisation assay, REBA Myco-ID®, was applied to formalin-fixed paraffin-embedded (FFPE) tissue showing granulomatous lymphadenitis to define the causative agents. DESIGN: A total of 119 granulomatous lymphadenitis cases observed between 2000 and 2010 were studied. All tissue samples were treated by haematoxylin and eosin and Ziehl-Neelsen stain. Mycobacterium tuberculosis and non-tuberculous mycobacteria (NTM) were identified using the REBA Myco-ID assay, and resistance to rifampicin (RMP) and isoniazid (INH) was determined using REBA MTB-MDR®. RESULTS: Of the 119 cases, 113 (95%) were positive with the REBA Myco-ID assay, while 20 (16.8%) were positive on acid-fast bacilli smear. Of the 113 positive REBA Myco-ID cases, 110 (92.43%) were identified as M. tuberculosis, 2 (1.7%) as NTM, and 1 (0.8%) as coinfection with M. tuberculosis and M. chelonae. Only 1 (0.9%) of the 110 M. tuberculosis cases was identified as RMP-resistant. CONCLUSION: REBA Myco-ID is a highly sensitive and specific assay for detecting M. tuberculosis and NTM. M. tuberculosis is the main cause of granulomatous lymphadenitis.

Original languageEnglish
Pages (from-to)898-902
Number of pages5
JournalInternational Journal of Tuberculosis and Lung Disease
Volume17
Issue number7
DOIs
Publication statusPublished - 2013 Jul 1

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Identification of Mycobacterium species in FFPE granulomatous lymphadenitis tissue using REBA Myco-ID®'. Together they form a unique fingerprint.

  • Cite this

    Munkhdelger, J., Wang, H. Y., Choi, Y., Wairagu, P. M., Lee, D., Park, S., Kim, S., Jeon, B. Y., Lee, H., & Park, K. H. (2013). Identification of Mycobacterium species in FFPE granulomatous lymphadenitis tissue using REBA Myco-ID®. International Journal of Tuberculosis and Lung Disease, 17(7), 898-902. https://doi.org/10.5588/ijtld.12.0818