Identification of Mycobacterium species in FFPE granulomatous lymphadenitis tissue using REBA Myco-ID®

J. Munkhdelger, H. Y. Wang, Y. Choi, P. M. Wairagu, D. Lee, S. Park, S. Kim, B. Y. Jeon, H. Lee, K. H. Park

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

SETTING: Tuberculous lymphadenitis is the most common form of lymphadenopathy; its main histopathological finding is granulomatous inflammation. OBJECTIVE: A reverse blot hybridisation assay, REBA Myco-ID®, was applied to formalin-fixed paraffin-embedded (FFPE) tissue showing granulomatous lymphadenitis to define the causative agents. DESIGN: A total of 119 granulomatous lymphadenitis cases observed between 2000 and 2010 were studied. All tissue samples were treated by haematoxylin and eosin and Ziehl-Neelsen stain. Mycobacterium tuberculosis and non-tuberculous mycobacteria (NTM) were identified using the REBA Myco-ID assay, and resistance to rifampicin (RMP) and isoniazid (INH) was determined using REBA MTB-MDR®. RESULTS: Of the 119 cases, 113 (95%) were positive with the REBA Myco-ID assay, while 20 (16.8%) were positive on acid-fast bacilli smear. Of the 113 positive REBA Myco-ID cases, 110 (92.43%) were identified as M. tuberculosis, 2 (1.7%) as NTM, and 1 (0.8%) as coinfection with M. tuberculosis and M. chelonae. Only 1 (0.9%) of the 110 M. tuberculosis cases was identified as RMP-resistant. CONCLUSION: REBA Myco-ID is a highly sensitive and specific assay for detecting M. tuberculosis and NTM. M. tuberculosis is the main cause of granulomatous lymphadenitis.

Original languageEnglish
Pages (from-to)898-902
Number of pages5
JournalInternational Journal of Tuberculosis and Lung Disease
Volume17
Issue number7
DOIs
Publication statusPublished - 2013 Jul 1

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Lymphadenitis
Mycobacterium
Mycobacterium tuberculosis
Paraffin
Formaldehyde
Rifampin
Lymph Node Tuberculosis
Isoniazid
Hematoxylin
Eosine Yellowish-(YS)
Coinfection
Bacillus
Coloring Agents
Inflammation
Acids

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

Cite this

Munkhdelger, J. ; Wang, H. Y. ; Choi, Y. ; Wairagu, P. M. ; Lee, D. ; Park, S. ; Kim, S. ; Jeon, B. Y. ; Lee, H. ; Park, K. H. / Identification of Mycobacterium species in FFPE granulomatous lymphadenitis tissue using REBA Myco-ID®. In: International Journal of Tuberculosis and Lung Disease. 2013 ; Vol. 17, No. 7. pp. 898-902.
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title = "Identification of Mycobacterium species in FFPE granulomatous lymphadenitis tissue using REBA Myco-ID{\circledR}",
abstract = "SETTING: Tuberculous lymphadenitis is the most common form of lymphadenopathy; its main histopathological finding is granulomatous inflammation. OBJECTIVE: A reverse blot hybridisation assay, REBA Myco-ID{\circledR}, was applied to formalin-fixed paraffin-embedded (FFPE) tissue showing granulomatous lymphadenitis to define the causative agents. DESIGN: A total of 119 granulomatous lymphadenitis cases observed between 2000 and 2010 were studied. All tissue samples were treated by haematoxylin and eosin and Ziehl-Neelsen stain. Mycobacterium tuberculosis and non-tuberculous mycobacteria (NTM) were identified using the REBA Myco-ID assay, and resistance to rifampicin (RMP) and isoniazid (INH) was determined using REBA MTB-MDR{\circledR}. RESULTS: Of the 119 cases, 113 (95{\%}) were positive with the REBA Myco-ID assay, while 20 (16.8{\%}) were positive on acid-fast bacilli smear. Of the 113 positive REBA Myco-ID cases, 110 (92.43{\%}) were identified as M. tuberculosis, 2 (1.7{\%}) as NTM, and 1 (0.8{\%}) as coinfection with M. tuberculosis and M. chelonae. Only 1 (0.9{\%}) of the 110 M. tuberculosis cases was identified as RMP-resistant. CONCLUSION: REBA Myco-ID is a highly sensitive and specific assay for detecting M. tuberculosis and NTM. M. tuberculosis is the main cause of granulomatous lymphadenitis.",
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Identification of Mycobacterium species in FFPE granulomatous lymphadenitis tissue using REBA Myco-ID®. / Munkhdelger, J.; Wang, H. Y.; Choi, Y.; Wairagu, P. M.; Lee, D.; Park, S.; Kim, S.; Jeon, B. Y.; Lee, H.; Park, K. H.

In: International Journal of Tuberculosis and Lung Disease, Vol. 17, No. 7, 01.07.2013, p. 898-902.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification of Mycobacterium species in FFPE granulomatous lymphadenitis tissue using REBA Myco-ID®

AU - Munkhdelger, J.

AU - Wang, H. Y.

AU - Choi, Y.

AU - Wairagu, P. M.

AU - Lee, D.

AU - Park, S.

AU - Kim, S.

AU - Jeon, B. Y.

AU - Lee, H.

AU - Park, K. H.

PY - 2013/7/1

Y1 - 2013/7/1

N2 - SETTING: Tuberculous lymphadenitis is the most common form of lymphadenopathy; its main histopathological finding is granulomatous inflammation. OBJECTIVE: A reverse blot hybridisation assay, REBA Myco-ID®, was applied to formalin-fixed paraffin-embedded (FFPE) tissue showing granulomatous lymphadenitis to define the causative agents. DESIGN: A total of 119 granulomatous lymphadenitis cases observed between 2000 and 2010 were studied. All tissue samples were treated by haematoxylin and eosin and Ziehl-Neelsen stain. Mycobacterium tuberculosis and non-tuberculous mycobacteria (NTM) were identified using the REBA Myco-ID assay, and resistance to rifampicin (RMP) and isoniazid (INH) was determined using REBA MTB-MDR®. RESULTS: Of the 119 cases, 113 (95%) were positive with the REBA Myco-ID assay, while 20 (16.8%) were positive on acid-fast bacilli smear. Of the 113 positive REBA Myco-ID cases, 110 (92.43%) were identified as M. tuberculosis, 2 (1.7%) as NTM, and 1 (0.8%) as coinfection with M. tuberculosis and M. chelonae. Only 1 (0.9%) of the 110 M. tuberculosis cases was identified as RMP-resistant. CONCLUSION: REBA Myco-ID is a highly sensitive and specific assay for detecting M. tuberculosis and NTM. M. tuberculosis is the main cause of granulomatous lymphadenitis.

AB - SETTING: Tuberculous lymphadenitis is the most common form of lymphadenopathy; its main histopathological finding is granulomatous inflammation. OBJECTIVE: A reverse blot hybridisation assay, REBA Myco-ID®, was applied to formalin-fixed paraffin-embedded (FFPE) tissue showing granulomatous lymphadenitis to define the causative agents. DESIGN: A total of 119 granulomatous lymphadenitis cases observed between 2000 and 2010 were studied. All tissue samples were treated by haematoxylin and eosin and Ziehl-Neelsen stain. Mycobacterium tuberculosis and non-tuberculous mycobacteria (NTM) were identified using the REBA Myco-ID assay, and resistance to rifampicin (RMP) and isoniazid (INH) was determined using REBA MTB-MDR®. RESULTS: Of the 119 cases, 113 (95%) were positive with the REBA Myco-ID assay, while 20 (16.8%) were positive on acid-fast bacilli smear. Of the 113 positive REBA Myco-ID cases, 110 (92.43%) were identified as M. tuberculosis, 2 (1.7%) as NTM, and 1 (0.8%) as coinfection with M. tuberculosis and M. chelonae. Only 1 (0.9%) of the 110 M. tuberculosis cases was identified as RMP-resistant. CONCLUSION: REBA Myco-ID is a highly sensitive and specific assay for detecting M. tuberculosis and NTM. M. tuberculosis is the main cause of granulomatous lymphadenitis.

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