Identification of novel allergenic components from German cockroach fecal extract by a proteomic approach

Kyoung Yong Jeong, Chung Ryul Kim, Jina Park, In Soo Han, Jungwon Park, Taisoon Yong

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background: Cockroaches produce potent allergens, and cockroach feces are known to be especially rich in allergens. In this study, we analyze the allergenic components from cockroach feces and evaluate allergenicity of recombinant α-amylase identified from fecal extract. Methods: IgE-reactive proteins from German cockroach fecal extract were analyzed by proteomic analysis and immunoblotting. Recombinant α-amylase was produced and its allergenicity was evaluated by ELISA. Results: Analysis of German cockroach fecal extracts identified 12 IgE-reactive components. Most of these allergens were found to be digestive enzymes such as α-amylase, trypsin, chymotrypsin, metalloprotease, and midgut carboxypeptidase A, but the identity of 3 IgE-reactive proteins is still unknown. Glycinin-like proteins, which were likely derived from the cockroach diet, were also identified. German cockroach α-amylase shares the highest identity with pig α-amylase (55.8%), followed by mite group 4 allergens (Blo t 4, 50.4%; Der p 4, 49.8%; Eur m 4, 47.4%). In this study, recombinant α-amylase from German cockroach was expressed, and its allergenicity was examined by ELISA. Specific IgE against recombinant amylase was detected in 41.4% (12/29) of serum samples from German cockroach-sensitized subjects. Recombinant α-amylase was able to inhibit 55% of specific IgE to German cockroach whole-body extract. Conclusions: Amylase was found to be an important novel allergen in cockroach feces. It is hoped that recombinant α-amylase will be useful for further studies and clinical applications.

Original languageEnglish
Pages (from-to)315-324
Number of pages10
JournalInternational Archives of Allergy and Immunology
Volume161
Issue number4
DOIs
Publication statusPublished - 2013 Jul 1

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Blattellidae
Amylases
Proteomics
Cockroaches
Allergens
Immunoglobulin E
Feces
Enzyme-Linked Immunosorbent Assay
Carboxypeptidases A
Proteins
Mites
Metalloproteases
Immunoblotting
Swine

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

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title = "Identification of novel allergenic components from German cockroach fecal extract by a proteomic approach",
abstract = "Background: Cockroaches produce potent allergens, and cockroach feces are known to be especially rich in allergens. In this study, we analyze the allergenic components from cockroach feces and evaluate allergenicity of recombinant α-amylase identified from fecal extract. Methods: IgE-reactive proteins from German cockroach fecal extract were analyzed by proteomic analysis and immunoblotting. Recombinant α-amylase was produced and its allergenicity was evaluated by ELISA. Results: Analysis of German cockroach fecal extracts identified 12 IgE-reactive components. Most of these allergens were found to be digestive enzymes such as α-amylase, trypsin, chymotrypsin, metalloprotease, and midgut carboxypeptidase A, but the identity of 3 IgE-reactive proteins is still unknown. Glycinin-like proteins, which were likely derived from the cockroach diet, were also identified. German cockroach α-amylase shares the highest identity with pig α-amylase (55.8{\%}), followed by mite group 4 allergens (Blo t 4, 50.4{\%}; Der p 4, 49.8{\%}; Eur m 4, 47.4{\%}). In this study, recombinant α-amylase from German cockroach was expressed, and its allergenicity was examined by ELISA. Specific IgE against recombinant amylase was detected in 41.4{\%} (12/29) of serum samples from German cockroach-sensitized subjects. Recombinant α-amylase was able to inhibit 55{\%} of specific IgE to German cockroach whole-body extract. Conclusions: Amylase was found to be an important novel allergen in cockroach feces. It is hoped that recombinant α-amylase will be useful for further studies and clinical applications.",
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Identification of novel allergenic components from German cockroach fecal extract by a proteomic approach. / Jeong, Kyoung Yong; Kim, Chung Ryul; Park, Jina; Han, In Soo; Park, Jungwon; Yong, Taisoon.

In: International Archives of Allergy and Immunology, Vol. 161, No. 4, 01.07.2013, p. 315-324.

Research output: Contribution to journalArticle

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AU - Yong, Taisoon

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