Identification of parathyroid hormone-regulated proteins in mouse bone marrow cells by proteomics

Seong Hwan Kim, Sujung Jun, Hee Sun Jang, Sungkil Lim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The ability of parathyroid hormone (PTH) to enhance bone formation has recently been exploited in the treatment of osteoporosis. Several studies have suggested that the activation of bone marrow stromal cells could be preceded to show the anabolic effect of PTH on bone formation, but little is known of PTH-regulated proteins in bone marrow cells. Therefore, protein profiling in the intermittent PTH-treated bone marrow cells was evaluated using proteomics. Daily treatment for 5 days consisting of subcutaneous injection of either 150 μg/kg per day of mouse PTH (1-84) or vehicle (0.9% normal saline) was performed on the ICR mouse. At the end of the treatment period, bone marrow cells were separated and used in proteomics. The expression levels of seven proteins including vimentin were decreased, but those of four proteins including calreticulin and thioredoxin domain containing 7 protein (Txnde7) were increased. Among these, the decrease of vimentin and the increase of both calreticulin Txnde7 in mRNA levels were confirmed by semi-quantitative RT-PCR. In PTH-treated mouse MC3T3-E1 osteoblast cells, mRNA expression levels were not totally consistent with the results observed in proteomics. In conclusion, the differentially expressed proteins in bone marrow cells depending on PTH could be highly linked to the differentiation of osteoprogenitor cells in the bone marrow into preosteoblast cells.

Original languageEnglish
Pages (from-to)423-429
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume330
Issue number2
DOIs
Publication statusPublished - 2005 May 6

Fingerprint

Parathyroid Hormone
Bone Marrow Cells
Proteomics
Bone
Cells
Proteins
Calreticulin
Vimentin
Osteogenesis
Anabolic Agents
Inbred ICR Mouse
Messenger RNA
Thioredoxins
Subcutaneous Injections
Osteoblasts
Mesenchymal Stromal Cells
Osteoporosis
Therapeutics
Chemical activation
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "The ability of parathyroid hormone (PTH) to enhance bone formation has recently been exploited in the treatment of osteoporosis. Several studies have suggested that the activation of bone marrow stromal cells could be preceded to show the anabolic effect of PTH on bone formation, but little is known of PTH-regulated proteins in bone marrow cells. Therefore, protein profiling in the intermittent PTH-treated bone marrow cells was evaluated using proteomics. Daily treatment for 5 days consisting of subcutaneous injection of either 150 μg/kg per day of mouse PTH (1-84) or vehicle (0.9{\%} normal saline) was performed on the ICR mouse. At the end of the treatment period, bone marrow cells were separated and used in proteomics. The expression levels of seven proteins including vimentin were decreased, but those of four proteins including calreticulin and thioredoxin domain containing 7 protein (Txnde7) were increased. Among these, the decrease of vimentin and the increase of both calreticulin Txnde7 in mRNA levels were confirmed by semi-quantitative RT-PCR. In PTH-treated mouse MC3T3-E1 osteoblast cells, mRNA expression levels were not totally consistent with the results observed in proteomics. In conclusion, the differentially expressed proteins in bone marrow cells depending on PTH could be highly linked to the differentiation of osteoprogenitor cells in the bone marrow into preosteoblast cells.",
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Identification of parathyroid hormone-regulated proteins in mouse bone marrow cells by proteomics. / Kim, Seong Hwan; Jun, Sujung; Jang, Hee Sun; Lim, Sungkil.

In: Biochemical and Biophysical Research Communications, Vol. 330, No. 2, 06.05.2005, p. 423-429.

Research output: Contribution to journalArticle

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