Identification of PCR product by non-surface-binding method using label-free field-effect transistor

Jungim Han, Jeoyoung Shim, Wonseok Chung, Kyutae Yoo, Soosuk Lee, Euisik Yoon, Christopher Ko

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate an innovative method of PCR product identification that is based on thermal denaturation of PCR product-captured bead using integrated BioFET. Laborious purification and concentration of PCR product were easily carried by capturing the samples on the solid surface like microbeads. When temperature raised upto the denaturation point of PCR product, potential of BioFET is changed by mobile DNA in solution from PCR product-captured beads. By differentiating potential change with respect to temperature change, melting temperature can be determined. In this way, we successfully discriminated three kinds of PCR product using their different melting temperature and improved sensitivity of detection. We expect that proposed method can potentially be used for the identification of multiplex PCR products.

Original languageEnglish
Title of host publicationTechnical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008
Pages632-635
Number of pages4
Publication statusPublished - 2008 Oct 1
Event2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008 - Quebec City, QC, United States
Duration: 2008 Jun 12008 Jun 5

Publication series

NameTechnical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008
Volume2

Other

Other2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008
CountryUnited States
CityQuebec City, QC
Period08/6/108/6/5

Fingerprint

Denaturation
Field effect transistors
Melting point
Labels
Purification
DNA
Temperature
Hot Temperature

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering

Cite this

Han, J., Shim, J., Chung, W., Yoo, K., Lee, S., Yoon, E., & Ko, C. (2008). Identification of PCR product by non-surface-binding method using label-free field-effect transistor. In Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008 (pp. 632-635). (Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008; Vol. 2).
Han, Jungim ; Shim, Jeoyoung ; Chung, Wonseok ; Yoo, Kyutae ; Lee, Soosuk ; Yoon, Euisik ; Ko, Christopher. / Identification of PCR product by non-surface-binding method using label-free field-effect transistor. Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008. 2008. pp. 632-635 (Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008).
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abstract = "We demonstrate an innovative method of PCR product identification that is based on thermal denaturation of PCR product-captured bead using integrated BioFET. Laborious purification and concentration of PCR product were easily carried by capturing the samples on the solid surface like microbeads. When temperature raised upto the denaturation point of PCR product, potential of BioFET is changed by mobile DNA in solution from PCR product-captured beads. By differentiating potential change with respect to temperature change, melting temperature can be determined. In this way, we successfully discriminated three kinds of PCR product using their different melting temperature and improved sensitivity of detection. We expect that proposed method can potentially be used for the identification of multiplex PCR products.",
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Han, J, Shim, J, Chung, W, Yoo, K, Lee, S, Yoon, E & Ko, C 2008, Identification of PCR product by non-surface-binding method using label-free field-effect transistor. in Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008. Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008, vol. 2, pp. 632-635, 2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008, Quebec City, QC, United States, 08/6/1.

Identification of PCR product by non-surface-binding method using label-free field-effect transistor. / Han, Jungim; Shim, Jeoyoung; Chung, Wonseok; Yoo, Kyutae; Lee, Soosuk; Yoon, Euisik; Ko, Christopher.

Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008. 2008. p. 632-635 (Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Han J, Shim J, Chung W, Yoo K, Lee S, Yoon E et al. Identification of PCR product by non-surface-binding method using label-free field-effect transistor. In Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008. 2008. p. 632-635. (Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008).