Identification of the DNA sequence interacting with Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor 1

Junsoo Park, Myung Shin Lee, Seung Min Yoo, Wan Jeong Kwi, Daeyoup Lee, Joonho Choe, Taegun Seo

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiological agent of Kaposi's sarcoma. The open reading frame (K9) of KSHV encodes viral interferon regulatory factor 1 (vIRF1), which functions as a repressor of interferon-mediated signal transduction. The amino-terminal region of vIRF1 displays significant homology to the DNA-binding domain of cellular interferon regulatory factors, supporting the theory that the protein interacts with specific DNA sequences. Here, we identify the consensus sequence of vIRF1-binding sites from a pool of random oligonucleotides. Moreover, our data show that vIRF1 interacts with the K3:viral dihydrofolate reductase:viral interleukin 6 promoter region in the KSHV genome.

Original languageEnglish
Pages (from-to)12680-12684
Number of pages5
JournalJournal of Virology
Volume81
Issue number22
DOIs
Publication statusPublished - 2007 Nov 1

Fingerprint

Human herpesvirus 8
Human Herpesvirus 8
nucleotide sequences
Interferon Regulatory Factors
dihydrofolate reductase
Tetrahydrofolate Dehydrogenase
DNA-binding domains
consensus sequence
Kaposi's Sarcoma
etiological agents
Consensus Sequence
sarcoma
interferons
oligonucleotides
Genetic Promoter Regions
interleukin-6
Oligonucleotides
Interferons
Open Reading Frames
open reading frames

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Park, Junsoo ; Lee, Myung Shin ; Yoo, Seung Min ; Kwi, Wan Jeong ; Lee, Daeyoup ; Choe, Joonho ; Seo, Taegun. / Identification of the DNA sequence interacting with Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor 1. In: Journal of Virology. 2007 ; Vol. 81, No. 22. pp. 12680-12684.
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Identification of the DNA sequence interacting with Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor 1. / Park, Junsoo; Lee, Myung Shin; Yoo, Seung Min; Kwi, Wan Jeong; Lee, Daeyoup; Choe, Joonho; Seo, Taegun.

In: Journal of Virology, Vol. 81, No. 22, 01.11.2007, p. 12680-12684.

Research output: Contribution to journalArticle

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AB - Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiological agent of Kaposi's sarcoma. The open reading frame (K9) of KSHV encodes viral interferon regulatory factor 1 (vIRF1), which functions as a repressor of interferon-mediated signal transduction. The amino-terminal region of vIRF1 displays significant homology to the DNA-binding domain of cellular interferon regulatory factors, supporting the theory that the protein interacts with specific DNA sequences. Here, we identify the consensus sequence of vIRF1-binding sites from a pool of random oligonucleotides. Moreover, our data show that vIRF1 interacts with the K3:viral dihydrofolate reductase:viral interleukin 6 promoter region in the KSHV genome.

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