Identification of two-component regulatory genes involved in o-xylene degradation by Rhodococcus sp. strain DK17

Dockyu Kim, Jong Chan Chae, Gerben J. Zylstra, Ho Yong Sohn, Gi Seok Kwon, Eungbin Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17.

Original languageEnglish
Pages (from-to)49-53
Number of pages5
JournalJournal of Microbiology
Volume43
Issue number1
Publication statusPublished - 2005 Feb 1

Fingerprint

Rhodococcus
Dioxygenases
Regulator Genes
Operon
Histidine
Sequence Analysis
Signal Transduction
Adenosine Triphosphate
Polymerase Chain Reaction
Genes
2-xylene

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Kim, Dockyu ; Chae, Jong Chan ; Zylstra, Gerben J. ; Sohn, Ho Yong ; Kwon, Gi Seok ; Kim, Eungbin. / Identification of two-component regulatory genes involved in o-xylene degradation by Rhodococcus sp. strain DK17. In: Journal of Microbiology. 2005 ; Vol. 43, No. 1. pp. 49-53.
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Identification of two-component regulatory genes involved in o-xylene degradation by Rhodococcus sp. strain DK17. / Kim, Dockyu; Chae, Jong Chan; Zylstra, Gerben J.; Sohn, Ho Yong; Kwon, Gi Seok; Kim, Eungbin.

In: Journal of Microbiology, Vol. 43, No. 1, 01.02.2005, p. 49-53.

Research output: Contribution to journalArticle

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