Identification of two-component regulatory genes involved in o-xylene degradation by Rhodococcus sp. strain DK17

Dockyu Kim; Jong Chan Chae; Gerben J. Zylstra; Ho Yong Sohn; Gi Seok Kwon; Eungbin Kim

Identification of two-component regulatory genes involved in o-xylene degradation by Rhodococcus sp. strain DK17

Dockyu Kim, Jong Chan Chae, Gerben J. Zylstra, Ho Yong Sohn, Gi Seok Kwon, Eungbin Kim

Department of Systems Biology

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17.

Original language	English
Pages (from-to)	49-53
Number of pages	5
Journal	Journal of Microbiology
Volume	43
Issue number	1
Publication status	Published - 2005 Feb

All Science Journal Classification (ASJC) codes

Microbiology
Applied Microbiology and Biotechnology

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title = "Identification of two-component regulatory genes involved in o-xylene degradation by Rhodococcus sp. strain DK17",

abstract = "Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17.",

author = "Dockyu Kim and Chae, {Jong Chan} and Zylstra, {Gerben J.} and Sohn, {Ho Yong} and Kwon, {Gi Seok} and Eungbin Kim",

year = "2005",

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language = "English",

volume = "43",

pages = "49--53",

journal = "Journal of Microbiology",

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T1 - Identification of two-component regulatory genes involved in o-xylene degradation by Rhodococcus sp. strain DK17

AU - Kim, Dockyu

AU - Chae, Jong Chan

AU - Zylstra, Gerben J.

AU - Sohn, Ho Yong

AU - Kwon, Gi Seok

AU - Kim, Eungbin

PY - 2005/2

Y1 - 2005/2

N2 - Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17.

AB - Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17.

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JO - Journal of Microbiology

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