Abstract
Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17.
| Original language | English |
|---|---|
| Pages (from-to) | 49-53 |
| Number of pages | 5 |
| Journal | Journal of Microbiology |
| Volume | 43 |
| Issue number | 1 |
| Publication status | Published - 2005 Feb 1 |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Applied Microbiology and Biotechnology
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Identification of two-component regulatory genes involved in o-xylene degradation by Rhodococcus sp. strain DK17. / Kim, Dockyu; Chae, Jong Chan; Zylstra, Gerben J.; Sohn, Ho Yong; Kwon, Gi Seok; Kim, Eungbin.
In: Journal of Microbiology, Vol. 43, No. 1, 01.02.2005, p. 49-53.Research output: Contribution to journal › Article
TY - JOUR
T1 - Identification of two-component regulatory genes involved in o-xylene degradation by Rhodococcus sp. strain DK17
AU - Kim, Dockyu
AU - Chae, Jong Chan
AU - Zylstra, Gerben J.
AU - Sohn, Ho Yong
AU - Kwon, Gi Seok
AU - Kim, Eungbin
PY - 2005/2/1
Y1 - 2005/2/1
N2 - Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17.
AB - Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17.
UR - http://www.scopus.com/inward/record.url?scp=15944388209&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=15944388209&partnerID=8YFLogxK
M3 - Article
C2 - 15765058
AN - SCOPUS:15944388209
VL - 43
SP - 49
EP - 53
JO - Journal of Microbiology
JF - Journal of Microbiology
SN - 1225-8873
IS - 1
ER -