Identification of two-component regulatory genes involved in o-xylene degradation by Rhodococcus sp. strain DK17

Dockyu Kim, Jong Chan Chae, Gerben J. Zylstra, Ho Yong Sohn, Gi Seok Kwon, Eungbin Kim

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Abstract

Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17.

Original languageEnglish
Pages (from-to)49-53
Number of pages5
JournalJournal of Microbiology
Volume43
Issue number1
Publication statusPublished - 2005 Feb 1

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

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