IgE-binding epitope analysis of Bla g 5, the german cockroach allergen

Kyoung Jin Jeong, Kyoung Yong Jeong, Chung Ryul Kim, Tai Soon Yong

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cockroach infestations have been linked with allergic diseases such as asthma in humans. Bla g 5, sigma class glutathione S-transferase (GST), is the major cockroach allergen which has the highest IgE response value of all cockroach allergens. Although several cockroach allergens have been identified and cloned, information regarding their B ell and T cell IgE-binding epitopes is limited. In order to analyze the IgE binding epitopes of Bla g 5, full-length and five peptide fragments (A, 1-100 amino acid residue; B, 91-201; Ba, 1-125; Bb, 1-150; Bc, 1-175) were expressed. Twelve (37.5%) of 32 sera from cockroach-sensitized subjects showed positive IgE reactivity to the recombinant Bla g 5 (rBla g 5). Six strong positive sera were selected for the epitope study. Recombinant proteins not containing 176-201 amino acid residues were unable to react to sera from cockroach sensitized individuals, suggesting that this region contains the IgE-binding epitope. Despite strong IgE reactivity to rBla g 5, the pooled serum from 5 cockroach-sensitized patients did not show IgE reactivity to all synthetic peptides consisting of 15 residues covering 161-201 amino acids. These results suggest the possibility that Bla g 5 may have a conformational epitope in the C-terminal region. GST is the important target for the development of vaccines and drugs against allergic diseases because of high cross-reactivity among insect species. This study will aid recombinant allergen research for immunotherapy of cockroach allergens and other insect allergens.

Original languageEnglish
Pages (from-to)573-577
Number of pages5
JournalProtein and Peptide Letters
Volume17
Issue number5
DOIs
Publication statusPublished - 2010 May 13

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Blattellidae
Cockroaches
Allergens
Immunoglobulin E
Epitopes
Glutathione Transferase
Amino Acids
Serum
Insects
Peptide Fragments
Immunologic Desensitization
T-cells
Recombinant Proteins
Vaccines
Asthma
Peptides
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry

Cite this

Jeong, Kyoung Jin ; Jeong, Kyoung Yong ; Kim, Chung Ryul ; Yong, Tai Soon. / IgE-binding epitope analysis of Bla g 5, the german cockroach allergen. In: Protein and Peptide Letters. 2010 ; Vol. 17, No. 5. pp. 573-577.
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abstract = "Cockroach infestations have been linked with allergic diseases such as asthma in humans. Bla g 5, sigma class glutathione S-transferase (GST), is the major cockroach allergen which has the highest IgE response value of all cockroach allergens. Although several cockroach allergens have been identified and cloned, information regarding their B ell and T cell IgE-binding epitopes is limited. In order to analyze the IgE binding epitopes of Bla g 5, full-length and five peptide fragments (A, 1-100 amino acid residue; B, 91-201; Ba, 1-125; Bb, 1-150; Bc, 1-175) were expressed. Twelve (37.5{\%}) of 32 sera from cockroach-sensitized subjects showed positive IgE reactivity to the recombinant Bla g 5 (rBla g 5). Six strong positive sera were selected for the epitope study. Recombinant proteins not containing 176-201 amino acid residues were unable to react to sera from cockroach sensitized individuals, suggesting that this region contains the IgE-binding epitope. Despite strong IgE reactivity to rBla g 5, the pooled serum from 5 cockroach-sensitized patients did not show IgE reactivity to all synthetic peptides consisting of 15 residues covering 161-201 amino acids. These results suggest the possibility that Bla g 5 may have a conformational epitope in the C-terminal region. GST is the important target for the development of vaccines and drugs against allergic diseases because of high cross-reactivity among insect species. This study will aid recombinant allergen research for immunotherapy of cockroach allergens and other insect allergens.",
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IgE-binding epitope analysis of Bla g 5, the german cockroach allergen. / Jeong, Kyoung Jin; Jeong, Kyoung Yong; Kim, Chung Ryul; Yong, Tai Soon.

In: Protein and Peptide Letters, Vol. 17, No. 5, 13.05.2010, p. 573-577.

Research output: Contribution to journalArticle

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