IgE production in CD40/CD40L cross-talk of B and mast cells and mediator release via TGase 2 in mouse allergic asthma

Gwan Ui Hong, Bum Soo Park, Jung Won Park, Soo Youl Kim, Jai Youl Ro

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)


TGase 2 is over-expressed in a variety of inflammatory diseases including allergic asthma. This study aimed to investigate the role of TGase 2 on IgE production and signaling pathways in mast cell activation related to OVA-induced allergic asthma. Bone marrow-derived mast cells (BMMCs) isolated from WT or TGase 2-/- mice were activated with Ag/Ab (refer to act-WT-BMMCs and act-KO-BMMCs, respectively). B cells isolated from splenocytes were activated with anti-mouse IgM (act-B cells), and B cells were co-cultured with BMMCs. WT and TGase 2-/- mice were sensitized and challenged with OVA adsorbed in alum hydroxide. Intracellular Ca2+ ([Ca2+]i) levels were determined by fluorescence intensity; IgE, mediators and TGase 2 activity by ELISA; the CD138 expression by FACS analyzer; cell surface markers and signal molecules by Western blot; NF-κB by EMSA; co-localization of mast cells and B cells by immunohistochemistry; Fcε RI-mediated mast cell activation by PCA test; expression of cytokines, MMPs, TIMPs, TLR2 and FceRI by RT-PCR. In vitro, act-KO-BMMCs reduced the [Ca2+]i levels, NF-κB activity, expression of CD40/CD40L, plasma cells, total IgE levels and TGase 2 activity in act-B cells co-cultured with act-BMMCs, expression of inflammatory cytokines and MMPs2/9, release of mediators (TNF-α, LTs and cytokines), and activities of signal molecules (PKCs, MAP kinases, I-κB and PLA2), which were all increased in act-WT-BMMCs. TGase 2 siRNA transfected/activated-BMMCs reduced all responses as same as those in act-KO-BMMCs. In allergic asthma model, TGase 2-/- mice protected against PCA reaction, OVA-specific IgE production and AHR, and they reduced co-localization of mast cells and B cells or IgE in lung tissues, expression and co-localization of surface molecules in mast cells (c-kit and CD40L) and B cells (CD23 and CD40), inflammatory cells including mast cells, goblet cells, amounts of collagen and mediator release in BAL fluid and/or lung tissues, which were all increased in WT mice. TLR expression in TGase 2-/- mice did not differ from those in WT mice. Our data suggest that TGase 2 expression and Ca2+ influx required by bidirectional events in mast cell activation facilitate IgE production in B cells via up-regulating mast cell CD40L expression, and induce the expression of numerous signaling molecules associated with airway inflammation and remodeling in allergic asthma.

Original languageEnglish
Pages (from-to)1514-1525
Number of pages12
JournalCellular Signalling
Issue number6
Publication statusPublished - 2013 Jun

Bibliographical note

Funding Information:
This study was supported by Samsung Biomedical Research Institute grant (# GL1B31411 ) to J.Y. Ro, and partially supported by a National Research Foundation grant (no. 2010-0029919 ) funded by the Korea Government (MEST) to S.Y. Kim.

All Science Journal Classification (ASJC) codes

  • Cell Biology


Dive into the research topics of 'IgE production in CD40/CD40L cross-talk of B and mast cells and mediator release via TGase 2 in mouse allergic asthma'. Together they form a unique fingerprint.

Cite this