IGF-1 counteracts TGF-β-mediated enhancement of fibronectin for in vitro human lens epithelial cells

So Hyang Chung, Sun Ah Jung, Young Jae Cho, Joon H. Lee, Eung Kweon Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purpose: To determine whether insulin like growth factor (IGF-1) affects transforming growth factor (TGF-β)-mediated fibronectin accumulation in human lens epithelial cell line (BLE B-3) cells. Materials and Methods: HLE B-3 cells were incubated for 24 hours with TGF-β (10 ng/mL), IGF-1 (10 ng/mL), or both. Expression of the fibronectin gene was determined using a real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Fibronectin levels were examined using western blot analysis and immunofluorescence staining. Result: Expression of the fibronectin gene was not different between the TGF-β/IGF-1 treated group and the TGF-β treated group (p = 0.116). However, western blot analysis demonstrated decreased fibronectin levels in human lens epithelial cells treated with TGF-β and IGF-1 compared to those treated with TGF-β only (p<0.01). Immunofluorescence staining disclosed inhibition of TGF-β-induced fibronectin in the presence of IGF-1. Conclusion: This study suggests that IGF-1 counteracts TGF-β-mediated fibronectin accumulation in human lens epithelial cells.

Original languageEnglish
Pages (from-to)949-954
Number of pages6
JournalYonsei medical journal
Volume48
Issue number6
DOIs
Publication statusPublished - 2007 Dec 1

Fingerprint

Insulin-Like Growth Factor I
Fibronectins
Lenses
Epithelial Cells
Fluorescent Antibody Technique
Western Blotting
Staining and Labeling
Gene Expression
Transforming Growth Factors
Somatomedins
In Vitro Techniques
Reverse Transcriptase Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Cell Line

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Chung, So Hyang ; Jung, Sun Ah ; Cho, Young Jae ; Lee, Joon H. ; Kim, Eung Kweon. / IGF-1 counteracts TGF-β-mediated enhancement of fibronectin for in vitro human lens epithelial cells. In: Yonsei medical journal. 2007 ; Vol. 48, No. 6. pp. 949-954.
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abstract = "Purpose: To determine whether insulin like growth factor (IGF-1) affects transforming growth factor (TGF-β)-mediated fibronectin accumulation in human lens epithelial cell line (BLE B-3) cells. Materials and Methods: HLE B-3 cells were incubated for 24 hours with TGF-β (10 ng/mL), IGF-1 (10 ng/mL), or both. Expression of the fibronectin gene was determined using a real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Fibronectin levels were examined using western blot analysis and immunofluorescence staining. Result: Expression of the fibronectin gene was not different between the TGF-β/IGF-1 treated group and the TGF-β treated group (p = 0.116). However, western blot analysis demonstrated decreased fibronectin levels in human lens epithelial cells treated with TGF-β and IGF-1 compared to those treated with TGF-β only (p<0.01). Immunofluorescence staining disclosed inhibition of TGF-β-induced fibronectin in the presence of IGF-1. Conclusion: This study suggests that IGF-1 counteracts TGF-β-mediated fibronectin accumulation in human lens epithelial cells.",
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IGF-1 counteracts TGF-β-mediated enhancement of fibronectin for in vitro human lens epithelial cells. / Chung, So Hyang; Jung, Sun Ah; Cho, Young Jae; Lee, Joon H.; Kim, Eung Kweon.

In: Yonsei medical journal, Vol. 48, No. 6, 01.12.2007, p. 949-954.

Research output: Contribution to journalArticle

TY - JOUR

T1 - IGF-1 counteracts TGF-β-mediated enhancement of fibronectin for in vitro human lens epithelial cells

AU - Chung, So Hyang

AU - Jung, Sun Ah

AU - Cho, Young Jae

AU - Lee, Joon H.

AU - Kim, Eung Kweon

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N2 - Purpose: To determine whether insulin like growth factor (IGF-1) affects transforming growth factor (TGF-β)-mediated fibronectin accumulation in human lens epithelial cell line (BLE B-3) cells. Materials and Methods: HLE B-3 cells were incubated for 24 hours with TGF-β (10 ng/mL), IGF-1 (10 ng/mL), or both. Expression of the fibronectin gene was determined using a real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Fibronectin levels were examined using western blot analysis and immunofluorescence staining. Result: Expression of the fibronectin gene was not different between the TGF-β/IGF-1 treated group and the TGF-β treated group (p = 0.116). However, western blot analysis demonstrated decreased fibronectin levels in human lens epithelial cells treated with TGF-β and IGF-1 compared to those treated with TGF-β only (p<0.01). Immunofluorescence staining disclosed inhibition of TGF-β-induced fibronectin in the presence of IGF-1. Conclusion: This study suggests that IGF-1 counteracts TGF-β-mediated fibronectin accumulation in human lens epithelial cells.

AB - Purpose: To determine whether insulin like growth factor (IGF-1) affects transforming growth factor (TGF-β)-mediated fibronectin accumulation in human lens epithelial cell line (BLE B-3) cells. Materials and Methods: HLE B-3 cells were incubated for 24 hours with TGF-β (10 ng/mL), IGF-1 (10 ng/mL), or both. Expression of the fibronectin gene was determined using a real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Fibronectin levels were examined using western blot analysis and immunofluorescence staining. Result: Expression of the fibronectin gene was not different between the TGF-β/IGF-1 treated group and the TGF-β treated group (p = 0.116). However, western blot analysis demonstrated decreased fibronectin levels in human lens epithelial cells treated with TGF-β and IGF-1 compared to those treated with TGF-β only (p<0.01). Immunofluorescence staining disclosed inhibition of TGF-β-induced fibronectin in the presence of IGF-1. Conclusion: This study suggests that IGF-1 counteracts TGF-β-mediated fibronectin accumulation in human lens epithelial cells.

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