IL-12 Priming during In Vitro Antigenic Stimulation Changes Properties of CD8 T Cells and Increases Generation of Effector and Memory Cells

Jun Chang, Jae Ho Cho, Seung Woo Lee, So Young Choi, Sang-Jun Ha, Young Chul Sang

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Antigenic and costimulatory signals trigger a developmental program by which naive CD8 T cells differentiate into effector and memory cells. However, initial cytokine signals that regulate the generation of effector and memory CD8 T cells are not well understood. In this study, we show that IL-12 priming during in vitro antigenic stimulation results in the significant increase of both primary and memory CD8 T cell population in mice after adoptive transfer of activated cells. The effect of IL-12 priming is closely associated with qualitative changes in CD8 T cells, such as reduced MHC I tetramer binding and CD69 expression, altered distribution of lipid rafts, decreased cytolytic activity, and less susceptibility to apoptosis. Furthermore, exogenous IL-12 priming improved the intrinsic survival properties of memory CD8 T cells, leading to better protective immunity and vaccine-induced memory CD8 T cell responses. However, the experiments with IL-12p40- and IL-12Rβ1-deficient mice showed similar levels of primary and memory CD8 T cell responses compared with wild-type mice, implying that endogenous IL-12 and/or IL-12R signaling in vivo is not critical for CD8 T cell immunity. Together, our results suggest that IL-12 can serve as an important, but dispensable regulatory factor for the development of CD8 T cells, and IL-12 priming could be useful in many medical applications.

Original languageEnglish
Pages (from-to)2818-2826
Number of pages9
JournalJournal of Immunology
Volume172
Issue number5
DOIs
Publication statusPublished - 2004 Mar 1

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Interleukin-12
T-Lymphocytes
Immunity
Interleukin-12 Subunit p40
In Vitro Techniques
Adoptive Transfer
Vaccines
Apoptosis
Cytokines
Lipids

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Chang, Jun ; Cho, Jae Ho ; Lee, Seung Woo ; Choi, So Young ; Ha, Sang-Jun ; Sang, Young Chul. / IL-12 Priming during In Vitro Antigenic Stimulation Changes Properties of CD8 T Cells and Increases Generation of Effector and Memory Cells. In: Journal of Immunology. 2004 ; Vol. 172, No. 5. pp. 2818-2826.
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IL-12 Priming during In Vitro Antigenic Stimulation Changes Properties of CD8 T Cells and Increases Generation of Effector and Memory Cells. / Chang, Jun; Cho, Jae Ho; Lee, Seung Woo; Choi, So Young; Ha, Sang-Jun; Sang, Young Chul.

In: Journal of Immunology, Vol. 172, No. 5, 01.03.2004, p. 2818-2826.

Research output: Contribution to journalArticle

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