IL-6 induces long-term protective immunity against a lethal challenge of influenza virus

Seung Woo Leethese Authors Equally Contributed To This Work., Jin Won Youn, Baik Lin Seong, Young Chul Sung

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The coadministration of cytokines can modulate immunity in DNA based viral vaccines. In order to determine the effects of various cytokines on long-term protection against the influenza virus, mice were intramuscularly coinoculated with plasmids that encoded either the granulocyte-macrophage colony-stimulating factor (GMCSF), interleukin-4 (IL-4), interleukin-12 (IL- 12), or the interleukin-6 (IL-6) gene, in the presence of two plasmids that encoded the nucleoprotein (NP) and the hemagglutinin (HA) gene of the influenza A virus. The coadministration of IL-4, IL-6 and IL-12 transiently enhanced antibody responses against influenza virus in early time points (4 to 7 week post immunization) after post inoculation. The expression of GMCSF gene resulted in the sustained elevation of antibody responses for at least 20 weeks post inoculation. However, NP-specific CTL responses decreased in these animals. Mice that received either the IL-12 or the IL-6 gene had enhanced NP-specific CTL responses. Remarkably, the coadministration of the IL-6 gene completely protected mice from a lethal challenge with influenza virus. Conversely, mice that received the IL-4 gene appeared to be more susceptible to lethal challenge than mice that were inoculated with the NP and the HA genes alone. These results demonstrate that the use of cytokines as molecular adjuvants when coadministered in influenza DNA vaccination must be specific. Our data also demonstrates that the coadministration of IL-6 should be considered to enhance the efficacy of influenza DNA vaccines.

Original languageEnglish
Pages (from-to)490-496
Number of pages7
JournalVaccine
Volume17
Issue number5
DOIs
Publication statusPublished - 1999 Feb 1

Fingerprint

Orthomyxoviridae
lethal genes
interleukin-6
Immunity
Interleukin-6
immunity
nucleoproteins
Nucleoproteins
interleukin-12
Genes
interleukin-4
Interleukin-12
Interleukin-4
mice
genes
granulocyte-macrophage colony-stimulating factor
cytokines
vaccination
Hemagglutinins
hemagglutinins

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Leethese Authors Equally Contributed To This Work., S. W., Youn, J. W., Seong, B. L., & Sung, Y. C. (1999). IL-6 induces long-term protective immunity against a lethal challenge of influenza virus. Vaccine, 17(5), 490-496. https://doi.org/10.1016/S0264-410X(98)00223-0
Leethese Authors Equally Contributed To This Work., Seung Woo ; Youn, Jin Won ; Seong, Baik Lin ; Sung, Young Chul. / IL-6 induces long-term protective immunity against a lethal challenge of influenza virus. In: Vaccine. 1999 ; Vol. 17, No. 5. pp. 490-496.
@article{c7db51b281ab460f91a48fa29597ce54,
title = "IL-6 induces long-term protective immunity against a lethal challenge of influenza virus",
abstract = "The coadministration of cytokines can modulate immunity in DNA based viral vaccines. In order to determine the effects of various cytokines on long-term protection against the influenza virus, mice were intramuscularly coinoculated with plasmids that encoded either the granulocyte-macrophage colony-stimulating factor (GMCSF), interleukin-4 (IL-4), interleukin-12 (IL- 12), or the interleukin-6 (IL-6) gene, in the presence of two plasmids that encoded the nucleoprotein (NP) and the hemagglutinin (HA) gene of the influenza A virus. The coadministration of IL-4, IL-6 and IL-12 transiently enhanced antibody responses against influenza virus in early time points (4 to 7 week post immunization) after post inoculation. The expression of GMCSF gene resulted in the sustained elevation of antibody responses for at least 20 weeks post inoculation. However, NP-specific CTL responses decreased in these animals. Mice that received either the IL-12 or the IL-6 gene had enhanced NP-specific CTL responses. Remarkably, the coadministration of the IL-6 gene completely protected mice from a lethal challenge with influenza virus. Conversely, mice that received the IL-4 gene appeared to be more susceptible to lethal challenge than mice that were inoculated with the NP and the HA genes alone. These results demonstrate that the use of cytokines as molecular adjuvants when coadministered in influenza DNA vaccination must be specific. Our data also demonstrates that the coadministration of IL-6 should be considered to enhance the efficacy of influenza DNA vaccines.",
author = "{Leethese Authors Equally Contributed To This Work.}, {Seung Woo} and Youn, {Jin Won} and Seong, {Baik Lin} and Sung, {Young Chul}",
year = "1999",
month = "2",
day = "1",
doi = "10.1016/S0264-410X(98)00223-0",
language = "English",
volume = "17",
pages = "490--496",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "5",

}

Leethese Authors Equally Contributed To This Work., SW, Youn, JW, Seong, BL & Sung, YC 1999, 'IL-6 induces long-term protective immunity against a lethal challenge of influenza virus', Vaccine, vol. 17, no. 5, pp. 490-496. https://doi.org/10.1016/S0264-410X(98)00223-0

IL-6 induces long-term protective immunity against a lethal challenge of influenza virus. / Leethese Authors Equally Contributed To This Work., Seung Woo; Youn, Jin Won; Seong, Baik Lin; Sung, Young Chul.

In: Vaccine, Vol. 17, No. 5, 01.02.1999, p. 490-496.

Research output: Contribution to journalArticle

TY - JOUR

T1 - IL-6 induces long-term protective immunity against a lethal challenge of influenza virus

AU - Leethese Authors Equally Contributed To This Work., Seung Woo

AU - Youn, Jin Won

AU - Seong, Baik Lin

AU - Sung, Young Chul

PY - 1999/2/1

Y1 - 1999/2/1

N2 - The coadministration of cytokines can modulate immunity in DNA based viral vaccines. In order to determine the effects of various cytokines on long-term protection against the influenza virus, mice were intramuscularly coinoculated with plasmids that encoded either the granulocyte-macrophage colony-stimulating factor (GMCSF), interleukin-4 (IL-4), interleukin-12 (IL- 12), or the interleukin-6 (IL-6) gene, in the presence of two plasmids that encoded the nucleoprotein (NP) and the hemagglutinin (HA) gene of the influenza A virus. The coadministration of IL-4, IL-6 and IL-12 transiently enhanced antibody responses against influenza virus in early time points (4 to 7 week post immunization) after post inoculation. The expression of GMCSF gene resulted in the sustained elevation of antibody responses for at least 20 weeks post inoculation. However, NP-specific CTL responses decreased in these animals. Mice that received either the IL-12 or the IL-6 gene had enhanced NP-specific CTL responses. Remarkably, the coadministration of the IL-6 gene completely protected mice from a lethal challenge with influenza virus. Conversely, mice that received the IL-4 gene appeared to be more susceptible to lethal challenge than mice that were inoculated with the NP and the HA genes alone. These results demonstrate that the use of cytokines as molecular adjuvants when coadministered in influenza DNA vaccination must be specific. Our data also demonstrates that the coadministration of IL-6 should be considered to enhance the efficacy of influenza DNA vaccines.

AB - The coadministration of cytokines can modulate immunity in DNA based viral vaccines. In order to determine the effects of various cytokines on long-term protection against the influenza virus, mice were intramuscularly coinoculated with plasmids that encoded either the granulocyte-macrophage colony-stimulating factor (GMCSF), interleukin-4 (IL-4), interleukin-12 (IL- 12), or the interleukin-6 (IL-6) gene, in the presence of two plasmids that encoded the nucleoprotein (NP) and the hemagglutinin (HA) gene of the influenza A virus. The coadministration of IL-4, IL-6 and IL-12 transiently enhanced antibody responses against influenza virus in early time points (4 to 7 week post immunization) after post inoculation. The expression of GMCSF gene resulted in the sustained elevation of antibody responses for at least 20 weeks post inoculation. However, NP-specific CTL responses decreased in these animals. Mice that received either the IL-12 or the IL-6 gene had enhanced NP-specific CTL responses. Remarkably, the coadministration of the IL-6 gene completely protected mice from a lethal challenge with influenza virus. Conversely, mice that received the IL-4 gene appeared to be more susceptible to lethal challenge than mice that were inoculated with the NP and the HA genes alone. These results demonstrate that the use of cytokines as molecular adjuvants when coadministered in influenza DNA vaccination must be specific. Our data also demonstrates that the coadministration of IL-6 should be considered to enhance the efficacy of influenza DNA vaccines.

UR - http://www.scopus.com/inward/record.url?scp=0344197021&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344197021&partnerID=8YFLogxK

U2 - 10.1016/S0264-410X(98)00223-0

DO - 10.1016/S0264-410X(98)00223-0

M3 - Article

VL - 17

SP - 490

EP - 496

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 5

ER -

Leethese Authors Equally Contributed To This Work. SW, Youn JW, Seong BL, Sung YC. IL-6 induces long-term protective immunity against a lethal challenge of influenza virus. Vaccine. 1999 Feb 1;17(5):490-496. https://doi.org/10.1016/S0264-410X(98)00223-0