IL-7 receptor deletion ameliorates diet-induced obesity and insulin resistance in mice

Miyoung Lee, Su Jin Song, Myung Sook Choi, Rina Yu, Taesun Park

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Abstract

Aim/hypothesis: Obesity-induced inflammation plays an important role in the development of insulin resistance and type 2 diabetes. Recent studies have demonstrated that adiposity can be improved by ablating certain inflammatory signalling pathways. Although the IL-7 receptor (IL-7R) is mostly known as a key regulator of T lymphocyte development and homeostasis, its role in obesity and metabolic diseases is unknown. Because IL-7 is markedly increased in the serum of obese individuals and IL-7R (also known as IL7R) is overexpressed in white adipose tissue (WAT) in obesity, we studied the metabolic consequences of genetic Il-7r ablation in mice. Methods: Age-matched Il-7r-deficient (Il-7r KO) and wild-type (WT) littermates were fed a standard chow or high-fat diet (HFD) for 14 weeks. Their serum metabolic variables were measured. The expression of genes and proteins related to insulin resistance and inflammation was evaluated in WAT. Results: We demonstrated that Il-7r KO mice exhibited significantly reduced body weight gain and visceral adiposity compared with WT controls on both chow and HFD. The expression of signalling molecules involved in adipogenesis was reduced in the WAT of Il-7r KO mice. We also found that Il-7r KO mice had significantly enhanced glucose homeostasis and insulin sensitivity. Consistent with an improved metabolic phenotype, proinflammatory cytokine production and macrophage infiltration was attenuated in the WAT of Il-7r KO mice. Conclusions/interpretation: The IL-7R plays an important role in the induction of HFD-induced adipogenesis and insulin resistance in mice.

Original languageEnglish
Pages (from-to)2361-2370
Number of pages10
JournalDiabetologia
Volume58
Issue number10
DOIs
Publication statusPublished - 2015 Oct 24

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Interleukin-7 Receptors
Insulin Resistance
White Adipose Tissue
Obesity
Diet
High Fat Diet
Adipogenesis
Adiposity
Homeostasis
Inflammation
Interleukin-7
Metabolic Diseases
Serum
Type 2 Diabetes Mellitus
Weight Gain
Macrophages
Body Weight
Cytokines
T-Lymphocytes
Phenotype

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Lee, Miyoung ; Song, Su Jin ; Choi, Myung Sook ; Yu, Rina ; Park, Taesun. / IL-7 receptor deletion ameliorates diet-induced obesity and insulin resistance in mice. In: Diabetologia. 2015 ; Vol. 58, No. 10. pp. 2361-2370.
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IL-7 receptor deletion ameliorates diet-induced obesity and insulin resistance in mice. / Lee, Miyoung; Song, Su Jin; Choi, Myung Sook; Yu, Rina; Park, Taesun.

In: Diabetologia, Vol. 58, No. 10, 24.10.2015, p. 2361-2370.

Research output: Contribution to journalArticle

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AU - Song, Su Jin

AU - Choi, Myung Sook

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