Imaging and Differentiation of Retinal Ganglion Cells in Ex Vivo Experimental Optic Nerve Degeneration by Differential Interference Contrast Microscopy

Juyeong Oh, Yu Jeong Kim, Youngho Cho, Subeen Park, Hyung Min Kim, Chulki Kim, Taikjin Lee, Seong Chan Jun, Ki Ho Park, Dae Yu Kim, Jae Hun Kim, Seok Hwan Kim

Research output: Contribution to journalArticle

Abstract

Purpose: Apoptotic loss of retinal ganglion cells (RGCs) is involved in various optic neuropathies, and its extent is closely related to visual impairment. Direct imaging and counting of RGCs is beneficial to the evaluation of RGC loss, but these processes are challenging with the conventional techniques, due to the transparency and hypo-reflectivity of RGCs as light-transmitting structures of the retina. Differential interference contrast (DIC) microscopy, which can provide real-time images of transparent specimens, is utilized to image neuronal cells including RGCs in the ganglion cell layer (GCL). Methods: Herein, we show that the neuronal cells within each GCL in an explanted rat retina, including the inner nuclear layer and the outer nuclear layer, can be imaged selectively by transmission-type DIC microscopy. RGCs were also differentiated from non-RGCs by the objective method. Results: RGCs were differentiated from non-RGCs in the GCL by their morphological features on DIC images with the aid of retrograde fluorescence labeling. Loss of RGCs was detected in optic-nerve-transection and retinal-ischemia-reperfusion models by DIC imaging. The images obtained from the reflection-type DIC microscopy were comparable to those from the transmission-type DIC microscopy. Conclusions: This method enables direct optical visualization of RGCs in experimental optic-nerve degeneration, thus providing the opportunity for more accurate evaluation of optic neuropathies as well as more effective investigation of diseases.

Original languageEnglish
Pages (from-to)760-769
Number of pages10
JournalCurrent Eye Research
Volume44
Issue number7
DOIs
Publication statusPublished - 2019 Jul 3

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Interference Microscopy
Nerve Degeneration
Retinal Ganglion Cells
Optic Nerve
Ganglia
Optic Nerve Diseases
Retina
Optic Nerve Injuries
Vision Disorders
Reperfusion
Ischemia
Fluorescence
Light

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Oh, Juyeong ; Kim, Yu Jeong ; Cho, Youngho ; Park, Subeen ; Kim, Hyung Min ; Kim, Chulki ; Lee, Taikjin ; Jun, Seong Chan ; Park, Ki Ho ; Kim, Dae Yu ; Kim, Jae Hun ; Kim, Seok Hwan. / Imaging and Differentiation of Retinal Ganglion Cells in Ex Vivo Experimental Optic Nerve Degeneration by Differential Interference Contrast Microscopy. In: Current Eye Research. 2019 ; Vol. 44, No. 7. pp. 760-769.
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Imaging and Differentiation of Retinal Ganglion Cells in Ex Vivo Experimental Optic Nerve Degeneration by Differential Interference Contrast Microscopy. / Oh, Juyeong; Kim, Yu Jeong; Cho, Youngho; Park, Subeen; Kim, Hyung Min; Kim, Chulki; Lee, Taikjin; Jun, Seong Chan; Park, Ki Ho; Kim, Dae Yu; Kim, Jae Hun; Kim, Seok Hwan.

In: Current Eye Research, Vol. 44, No. 7, 03.07.2019, p. 760-769.

Research output: Contribution to journalArticle

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AU - Oh, Juyeong

AU - Kim, Yu Jeong

AU - Cho, Youngho

AU - Park, Subeen

AU - Kim, Hyung Min

AU - Kim, Chulki

AU - Lee, Taikjin

AU - Jun, Seong Chan

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AU - Kim, Jae Hun

AU - Kim, Seok Hwan

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