Imaging and identification of waterborne parasites using a chip-scale microscope

Seung Ah Lee, Jessey Erath, Guoan Zheng, Xiaoze Ou, Phil Willems, Daniel Eichinger, Ana Rodriguez, Changhuei Yang

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We demonstrate a compact portable imaging system for the detection of waterborne parasites in resource-limited settings. The previously demonstrated sub-pixel sweeping microscopy (SPSM) technique is a lens-less imaging scheme that can achieve high-resolution (<1 μm) bright-field imaging over a large field-of-view (5.7 mmx4.3 mm). A chip-scale microscope system, based on the SPSM technique, can be used for automated and high-throughput imaging of protozoan parasite cysts for the effective diagnosis of waterborne enteric parasite infection. We successfully imaged and identified three major types of enteric parasite cysts, Giardia, Cryptosporidium, and Entamoeba, which can be found in fecal samples from infected patients. We believe that this compact imaging system can serve well as a diagnostic device in challenging environments, such as rural settings or emergency outbreaks.

Original languageEnglish
Article numbere89712
JournalPloS one
Volume9
Issue number2
DOIs
Publication statusPublished - 2014 Feb 26

Fingerprint

microscopes
Parasites
Microscopes
image analysis
Imaging techniques
parasites
Cysts
Microscopy
Imaging systems
Entamoeba
Giardia
Cryptosporidium
Microscopic examination
Parasitic Diseases
Pixels
microscopy
Lenses
Disease Outbreaks
Emergencies
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Lee, S. A., Erath, J., Zheng, G., Ou, X., Willems, P., Eichinger, D., ... Yang, C. (2014). Imaging and identification of waterborne parasites using a chip-scale microscope. PloS one, 9(2), [e89712]. https://doi.org/10.1371/journal.pone.0089712
Lee, Seung Ah ; Erath, Jessey ; Zheng, Guoan ; Ou, Xiaoze ; Willems, Phil ; Eichinger, Daniel ; Rodriguez, Ana ; Yang, Changhuei. / Imaging and identification of waterborne parasites using a chip-scale microscope. In: PloS one. 2014 ; Vol. 9, No. 2.
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Lee, SA, Erath, J, Zheng, G, Ou, X, Willems, P, Eichinger, D, Rodriguez, A & Yang, C 2014, 'Imaging and identification of waterborne parasites using a chip-scale microscope', PloS one, vol. 9, no. 2, e89712. https://doi.org/10.1371/journal.pone.0089712

Imaging and identification of waterborne parasites using a chip-scale microscope. / Lee, Seung Ah; Erath, Jessey; Zheng, Guoan; Ou, Xiaoze; Willems, Phil; Eichinger, Daniel; Rodriguez, Ana; Yang, Changhuei.

In: PloS one, Vol. 9, No. 2, e89712, 26.02.2014.

Research output: Contribution to journalArticle

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