Imaging and identification of waterborne parasites using a chip-scale microscope

Seung Ah Lee, Jessey Erath, Guoan Zheng, Xiaoze Ou, Phil Willems, Daniel Eichinger, Ana Rodriguez, Changhuei Yang

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We demonstrate a compact portable imaging system for the detection of waterborne parasites in resource-limited settings. The previously demonstrated sub-pixel sweeping microscopy (SPSM) technique is a lens-less imaging scheme that can achieve high-resolution (<1 μm) bright-field imaging over a large field-of-view (5.7 mmx4.3 mm). A chip-scale microscope system, based on the SPSM technique, can be used for automated and high-throughput imaging of protozoan parasite cysts for the effective diagnosis of waterborne enteric parasite infection. We successfully imaged and identified three major types of enteric parasite cysts, Giardia, Cryptosporidium, and Entamoeba, which can be found in fecal samples from infected patients. We believe that this compact imaging system can serve well as a diagnostic device in challenging environments, such as rural settings or emergency outbreaks.

Original languageEnglish
Article numbere89712
JournalPloS one
Volume9
Issue number2
DOIs
Publication statusPublished - 2014 Feb 26

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Lee, S. A., Erath, J., Zheng, G., Ou, X., Willems, P., Eichinger, D., Rodriguez, A., & Yang, C. (2014). Imaging and identification of waterborne parasites using a chip-scale microscope. PloS one, 9(2), [e89712]. https://doi.org/10.1371/journal.pone.0089712