Imaging based on random excitation of fluorescence localized by metallic nanoislands

Kyujung Kim, Youngjin Oh, Wonju Lee, Donghyun Kim

Research output: Contribution to journalConference article

Abstract

We investigated localized surface plasmon-based enhancement of spatial resolution for total internal reflection fluorescence microscopy. The improvement of spatial resolution is based on plasmon excitation localized by hot spots induced between nanoislands. Near-field distribution of electromagnetic waves on the surface was calculated. For experimental verification, silver nanoisland samples were fabricated using temperature annealing method on an SF10 glass substrate. Fluorescent images of fluorescent beads and biological molecules measured on nanoislands confirm the enhancement of imaging resolution relative to a control using thin film substrates.

Original languageEnglish
JournalOptics InfoBase Conference Papers
Publication statusPublished - 2010 Dec 1
EventLaser Science, LS 2010 - Rochester, NY, United States
Duration: 2010 Oct 242010 Oct 28

Fingerprint

spatial resolution
Fluorescence
Imaging techniques
fluorescence
augmentation
Fluorescence microscopy
Substrates
beads
Electromagnetic waves
excitation
near fields
electromagnetic radiation
Silver
silver
Annealing
microscopy
Glass
Thin films
Molecules
annealing

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

Cite this

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abstract = "We investigated localized surface plasmon-based enhancement of spatial resolution for total internal reflection fluorescence microscopy. The improvement of spatial resolution is based on plasmon excitation localized by hot spots induced between nanoislands. Near-field distribution of electromagnetic waves on the surface was calculated. For experimental verification, silver nanoisland samples were fabricated using temperature annealing method on an SF10 glass substrate. Fluorescent images of fluorescent beads and biological molecules measured on nanoislands confirm the enhancement of imaging resolution relative to a control using thin film substrates.",
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Imaging based on random excitation of fluorescence localized by metallic nanoislands. / Kim, Kyujung; Oh, Youngjin; Lee, Wonju; Kim, Donghyun.

In: Optics InfoBase Conference Papers, 01.12.2010.

Research output: Contribution to journalConference article

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AU - Kim, Kyujung

AU - Oh, Youngjin

AU - Lee, Wonju

AU - Kim, Donghyun

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Y1 - 2010/12/1

N2 - We investigated localized surface plasmon-based enhancement of spatial resolution for total internal reflection fluorescence microscopy. The improvement of spatial resolution is based on plasmon excitation localized by hot spots induced between nanoislands. Near-field distribution of electromagnetic waves on the surface was calculated. For experimental verification, silver nanoisland samples were fabricated using temperature annealing method on an SF10 glass substrate. Fluorescent images of fluorescent beads and biological molecules measured on nanoislands confirm the enhancement of imaging resolution relative to a control using thin film substrates.

AB - We investigated localized surface plasmon-based enhancement of spatial resolution for total internal reflection fluorescence microscopy. The improvement of spatial resolution is based on plasmon excitation localized by hot spots induced between nanoislands. Near-field distribution of electromagnetic waves on the surface was calculated. For experimental verification, silver nanoisland samples were fabricated using temperature annealing method on an SF10 glass substrate. Fluorescent images of fluorescent beads and biological molecules measured on nanoislands confirm the enhancement of imaging resolution relative to a control using thin film substrates.

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