Imaging intracellular viscosity of a single cell during photoinduced cell death

Marina K. Kuimova, Stanley W. Botchway, Anthony W. Parker, Milan Balaz, Hazel A. Collins, Harry L. Anderson, Klaus Suhling, Peter R. Ogilby

Research output: Contribution to journalArticle

345 Citations (Scopus)

Abstract

Diffusion-mediated cellular processes, such as metabolism, signalling and transport, depend on the hydrodynamic properties of the intracellular matrix. Photodynamic therapy, used in the treatment of cancer, relies on the generation of short-lived cytotoxic agents within a cell on irradiation of a drug. The efficacy of this treatment depends on the viscosity of the medium through which the cytotoxic agent must diffuse. Here, spectrally resolved fluorescence measurements of a porphyrin-dimer-based molecular rotor are used to quantify intracellular viscosity changes in single cells. We show that there is a dramatic increase in the viscosity of the immediate environment of the rotor on photoinduced cell death. The effect of this viscosity increase is observed directly in the diffusion-dependent kinetics of the photosensitized formation and decay of a key cytotoxic agent, singlet molecular oxygen. Using these tools, we provide insight into the dynamics of diffusion in cells, which is pertinent to drug delivery, cell signalling and intracellular mass transport.

Original languageEnglish
Pages (from-to)69-73
Number of pages5
JournalNature chemistry
Volume1
Issue number1
DOIs
Publication statusPublished - 2009 Apr 1

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Cell death
Cytotoxins
Viscosity
Imaging techniques
Rotors
Cell signaling
Photodynamic therapy
Molecular oxygen
Porphyrins
Drug delivery
Metabolism
Dimers
Mass transfer
Hydrodynamics
Fluorescence
Irradiation
Kinetics
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Kuimova, M. K., Botchway, S. W., Parker, A. W., Balaz, M., Collins, H. A., Anderson, H. L., ... Ogilby, P. R. (2009). Imaging intracellular viscosity of a single cell during photoinduced cell death. Nature chemistry, 1(1), 69-73. https://doi.org/10.1038/nchem.120
Kuimova, Marina K. ; Botchway, Stanley W. ; Parker, Anthony W. ; Balaz, Milan ; Collins, Hazel A. ; Anderson, Harry L. ; Suhling, Klaus ; Ogilby, Peter R. / Imaging intracellular viscosity of a single cell during photoinduced cell death. In: Nature chemistry. 2009 ; Vol. 1, No. 1. pp. 69-73.
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Kuimova, MK, Botchway, SW, Parker, AW, Balaz, M, Collins, HA, Anderson, HL, Suhling, K & Ogilby, PR 2009, 'Imaging intracellular viscosity of a single cell during photoinduced cell death', Nature chemistry, vol. 1, no. 1, pp. 69-73. https://doi.org/10.1038/nchem.120

Imaging intracellular viscosity of a single cell during photoinduced cell death. / Kuimova, Marina K.; Botchway, Stanley W.; Parker, Anthony W.; Balaz, Milan; Collins, Hazel A.; Anderson, Harry L.; Suhling, Klaus; Ogilby, Peter R.

In: Nature chemistry, Vol. 1, No. 1, 01.04.2009, p. 69-73.

Research output: Contribution to journalArticle

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Kuimova MK, Botchway SW, Parker AW, Balaz M, Collins HA, Anderson HL et al. Imaging intracellular viscosity of a single cell during photoinduced cell death. Nature chemistry. 2009 Apr 1;1(1):69-73. https://doi.org/10.1038/nchem.120