Abstract
We use stroboscopic quantitative phase microscopy to study cell deformation and the response to cavitation bubbles and transient shear stress resulting from laser-induced breakdown of an optically trapped nanoparticle. A bi-directional transient displacement of cytoplasm is observed during expansion and collapse of the cavitation bubble. In some cases, cell deformation is only observable at the microsecond time scale without any permanent change in cell shape or optical thickness. On a time scale of seconds, the cellular response to shear stress and cytoplasm deformation typically leads to retraction of the cellular edge most exposed to the flow, rounding of the cell body and, in some cases, loss of cellular dry mass. These results give a new insight into the cellular response to cavitation induced shear stress and related plasma membrane permeabilization. This study also demonstrates that laserinduced breakdown of a nanoparticle offers localized cavitation, which interacts with a single cell but without causing cell lysis.
| Original language | English |
|---|---|
| Article number | 120508 |
| Journal | Journal of Biomedical Optics |
| Volume | 16 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2011 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Biomedical Engineering
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Imaging the cellular response to transient shear stress using stroboscopic digital holography. / Antkowiak, Maciej; Arita, Yoshihiko; Dholakia, Kishan; Gunn-Moore, Frank.
In: Journal of Biomedical Optics, Vol. 16, No. 12, 120508, 01.01.2011.Research output: Contribution to journal › Article
TY - JOUR
T1 - Imaging the cellular response to transient shear stress using stroboscopic digital holography
AU - Antkowiak, Maciej
AU - Arita, Yoshihiko
AU - Dholakia, Kishan
AU - Gunn-Moore, Frank
PY - 2011/1/1
Y1 - 2011/1/1
N2 - We use stroboscopic quantitative phase microscopy to study cell deformation and the response to cavitation bubbles and transient shear stress resulting from laser-induced breakdown of an optically trapped nanoparticle. A bi-directional transient displacement of cytoplasm is observed during expansion and collapse of the cavitation bubble. In some cases, cell deformation is only observable at the microsecond time scale without any permanent change in cell shape or optical thickness. On a time scale of seconds, the cellular response to shear stress and cytoplasm deformation typically leads to retraction of the cellular edge most exposed to the flow, rounding of the cell body and, in some cases, loss of cellular dry mass. These results give a new insight into the cellular response to cavitation induced shear stress and related plasma membrane permeabilization. This study also demonstrates that laserinduced breakdown of a nanoparticle offers localized cavitation, which interacts with a single cell but without causing cell lysis.
AB - We use stroboscopic quantitative phase microscopy to study cell deformation and the response to cavitation bubbles and transient shear stress resulting from laser-induced breakdown of an optically trapped nanoparticle. A bi-directional transient displacement of cytoplasm is observed during expansion and collapse of the cavitation bubble. In some cases, cell deformation is only observable at the microsecond time scale without any permanent change in cell shape or optical thickness. On a time scale of seconds, the cellular response to shear stress and cytoplasm deformation typically leads to retraction of the cellular edge most exposed to the flow, rounding of the cell body and, in some cases, loss of cellular dry mass. These results give a new insight into the cellular response to cavitation induced shear stress and related plasma membrane permeabilization. This study also demonstrates that laserinduced breakdown of a nanoparticle offers localized cavitation, which interacts with a single cell but without causing cell lysis.
UR - http://www.scopus.com/inward/record.url?scp=84856680821&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84856680821&partnerID=8YFLogxK
U2 - 10.1117/1.3665441
DO - 10.1117/1.3665441
M3 - Article
C2 - 22191911
AN - SCOPUS:84856680821
VL - 16
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
SN - 1083-3668
IS - 12
M1 - 120508
ER -