Imaging the cellular response to transient shear stress using time-resolved digital holography

Yoshihiko Arita, Maciej Antkowiak, Frank Gunn-Moore, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Shear stress has been recognized as one of the biophysical methods by which to permeabilize plasma membranes of cells. In particular, high pressure transient hydrodynamic flows created by laser-induced cavitation have been shown to lead to the uptake of fluorophores and plasmid DNA. While the mechanism and dynamics of cavitation have been extensively studied using a variety of time-resolved imaging techniques, the cellular response to the cavitation bubble and cavitation induced transient hydrodynamic flows has never been shown in detail. We use time-resolved quantitative phase microscopy to study cellular response to laser-induced cavitation bubbles. Laser-induced breakdown of an optically trapped polystyrene nanoparticle (500nm in diameter) irradiated with a single nanosecond laser pulse at 532nm creates transient shear stress to surrounding cells without causing cell lysis. A bi-directional transient displacement of cytoplasm is observed during expansion and collapse of the cavitation bubble. In some cases, cell deformation is only observable at the microsecond time scale without any permanent change in cell shape or optical thickness. On a time scale of seconds, the cellular response to shear stress and cytoplasm deformation typically leads to retraction of the cellular edge most exposed to the flow, rounding of the cell body and, in some cases, loss of cellular dry mass. These results give a new insight into the cellular response to laser-induced shear stress and related plasma membrane permeabilization. This study also demonstrates that laser-induced breakdown of an optically trapped nanoparticle offers localized cavitation (70 μm in diameter), which interacts with a single cell.

Original languageEnglish
Title of host publicationOptical Elastography and Tissue Biomechanics
PublisherSPIE
ISBN (Print)9780819498595
DOIs
Publication statusPublished - 2014 Jan 1
EventOptical Elastography and Tissue Biomechanics - San Francisco, CA, United States
Duration: 2014 Feb 12014 Feb 2

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8946
ISSN (Print)1605-7422

Conference

ConferenceOptical Elastography and Tissue Biomechanics
CountryUnited States
CitySan Francisco, CA
Period14/2/114/2/2

Fingerprint

Holography
cavitation flow
Cavitation
holography
shear stress
Shear stress
Lasers
Imaging techniques
cells
Bubbles (in fluids)
lasers
Hydrodynamics
bubbles
cytoplasm
Nanoparticles
Cell membranes
Cytoplasm
Cell Membrane
breakdown
hydrodynamics

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Arita, Y., Antkowiak, M., Gunn-Moore, F., & Dholakia, K. (2014). Imaging the cellular response to transient shear stress using time-resolved digital holography. In Optical Elastography and Tissue Biomechanics [89460V] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8946). SPIE. https://doi.org/10.1117/12.2039973
Arita, Yoshihiko ; Antkowiak, Maciej ; Gunn-Moore, Frank ; Dholakia, Kishan. / Imaging the cellular response to transient shear stress using time-resolved digital holography. Optical Elastography and Tissue Biomechanics. SPIE, 2014. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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Arita, Y, Antkowiak, M, Gunn-Moore, F & Dholakia, K 2014, Imaging the cellular response to transient shear stress using time-resolved digital holography. in Optical Elastography and Tissue Biomechanics., 89460V, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8946, SPIE, Optical Elastography and Tissue Biomechanics, San Francisco, CA, United States, 14/2/1. https://doi.org/10.1117/12.2039973

Imaging the cellular response to transient shear stress using time-resolved digital holography. / Arita, Yoshihiko; Antkowiak, Maciej; Gunn-Moore, Frank; Dholakia, Kishan.

Optical Elastography and Tissue Biomechanics. SPIE, 2014. 89460V (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8946).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Arita Y, Antkowiak M, Gunn-Moore F, Dholakia K. Imaging the cellular response to transient shear stress using time-resolved digital holography. In Optical Elastography and Tissue Biomechanics. SPIE. 2014. 89460V. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2039973