Imidazolized magnetic nanovectors with endosome disrupting moieties for the intracellular delivery and imaging of siRNA

Eunjung Kim, Hwunjae Lee, Yonghee An, Eunji Jang, Eun Kyung Lim, Byunghoon Kang, Jin Suck Suh, Yong Min Huh, Seungjoo Haam

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The development of a synchronized delivery and imaging system for small interfering RNA (siRNA) is required for the clinical application of RNA interference (RNAi) in cancer treatment. Herein, we report a pH-responsive, magnetic nanoparticle-based siRNA delivery system that can facilitate the safe and efficient delivery and visualization of therapeutic siRNA by high-resolution magnetic resonance (MR) imaging. Cationic poly-l-lysine-graft-imidazole (PLI) with a reactive silane moiety was stably immobilized onto the surface of the assembled manganese ferrite nanoparticles (MFs) through an emulsion process, ensuring high water solubility, enhanced MR contrast effect, and endosome-disrupting functionality. The synthesized nanovectors were then complexed with siRNA targeting the CD44 gene via electrostatic interactions to verify the specific gene-silencing effect. The imidazolized magnetic nanovector (ImMNV) architectures developed here facilitated improved cellular internalization and exhibited a high level in vitro downregulation compared to non-imidazolized MNVs in metastatic breast cancer cells. This journal is

Original languageEnglish
Pages (from-to)8566-8575
Number of pages10
JournalJournal of Materials Chemistry B
Volume2
Issue number48
DOIs
Publication statusPublished - 2014 Dec 28

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)

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