Imipenem-EDTA disk method for differentiation of metallo-β-lactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp.

Dongeun Yong; Kyungwon Lee; Jong Hwa Yum; Hee Bong Shin; Gian Maria Rossolini; Yunsop Chong

doi:10.1128/JCM.40.10.3798-3801.2002

Imipenem-EDTA disk method for differentiation of metallo-β-lactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp.

Dongeun Yong, Kyungwon Lee, Jong Hwa Yum, Hee Bong Shin, Gian Maria Rossolini, Yunsop Chong

Department of Laboratory Medicine

Research output: Contribution to journal › Article

318 Citations (Scopus)

Abstract

Rapid detection of metallo-β-lactamase (MBL)-producing gram-negative bacilli is necessary to prevent their dissemination. The method using a disk with imipenem plus 750 μg of EDTA differentiated all MBL-producing pseudomonads, and the sensitivity and specificity for acinetobacters were 95.7 and 91.0%, respectively. The imipenem-EDTA disks were stable for 12 and 16 weeks at 4 and -20°C, respectively.

Original language	English
Pages (from-to)	3798-3801
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	40
Issue number	10
DOIs	https://doi.org/10.1128/JCM.40.10.3798-3801.2002
Publication status	Published - 2002 Oct 1

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All Science Journal Classification (ASJC) codes

Microbiology (medical)

Cite this

Yong, D., Lee, K., Yum, J. H., Shin, H. B., Rossolini, G. M., & Chong, Y. (2002). Imipenem-EDTA disk method for differentiation of metallo-β-lactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp. Journal of Clinical Microbiology, 40(10), 3798-3801. https://doi.org/10.1128/JCM.40.10.3798-3801.2002

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Imipenem-EDTA disk method for differentiation of metallo-β-lactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp. / Yong, Dongeun ; Lee, Kyungwon; Yum, Jong Hwa; Shin, Hee Bong; Rossolini, Gian Maria; Chong, Yunsop.

In: Journal of Clinical Microbiology, Vol. 40, No. 10, 01.10.2002, p. 3798-3801.

Research output: Contribution to journal › Article

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AU - Shin, Hee Bong

AU - Rossolini, Gian Maria

AU - Chong, Yunsop

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10.1128/JCM.40.10.3798-3801.2002