Imipenem-EDTA disk method for differentiation of metallo-β-lactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp.

Dongeun Yong, Kyungwon Lee, Jong Hwa Yum, Hee Bong Shin, Gian Maria Rossolini, Yunsop Chong

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Rapid detection of metallo-β-lactamase (MBL)-producing gram-negative bacilli is necessary to prevent their dissemination. The method using a disk with imipenem plus 750 μg of EDTA differentiated all MBL-producing pseudomonads, and the sensitivity and specificity for acinetobacters were 95.7 and 91.0%, respectively. The imipenem-EDTA disks were stable for 12 and 16 weeks at 4 and -20°C, respectively.

Original languageEnglish
Pages (from-to)3798-3801
Number of pages4
JournalJournal of Clinical Microbiology
Issue number10
Publication statusPublished - 2002 Oct 1


All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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