Imipenem-EDTA disk method for differentiation of metallo-β-lactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp.

Dongeun Yong, Kyungwon Lee, Jong Hwa Yum, Hee Bong Shin, Gian Maria Rossolini, Yunsop Chong

Research output: Contribution to journalArticle

306 Citations (Scopus)

Abstract

Rapid detection of metallo-β-lactamase (MBL)-producing gram-negative bacilli is necessary to prevent their dissemination. The method using a disk with imipenem plus 750 μg of EDTA differentiated all MBL-producing pseudomonads, and the sensitivity and specificity for acinetobacters were 95.7 and 91.0%, respectively. The imipenem-EDTA disks were stable for 12 and 16 weeks at 4 and -20°C, respectively.

Original languageEnglish
Pages (from-to)3798-3801
Number of pages4
JournalJournal of Clinical Microbiology
Volume40
Issue number10
DOIs
Publication statusPublished - 2002 Oct 1

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Acinetobacter
Imipenem
Pseudomonas
Edetic Acid
Bacillus
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

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title = "Imipenem-EDTA disk method for differentiation of metallo-β-lactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp.",
abstract = "Rapid detection of metallo-β-lactamase (MBL)-producing gram-negative bacilli is necessary to prevent their dissemination. The method using a disk with imipenem plus 750 μg of EDTA differentiated all MBL-producing pseudomonads, and the sensitivity and specificity for acinetobacters were 95.7 and 91.0{\%}, respectively. The imipenem-EDTA disks were stable for 12 and 16 weeks at 4 and -20°C, respectively.",
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Imipenem-EDTA disk method for differentiation of metallo-β-lactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp. / Yong, Dongeun; Lee, Kyungwon; Yum, Jong Hwa; Shin, Hee Bong; Rossolini, Gian Maria; Chong, Yunsop.

In: Journal of Clinical Microbiology, Vol. 40, No. 10, 01.10.2002, p. 3798-3801.

Research output: Contribution to journalArticle

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T1 - Imipenem-EDTA disk method for differentiation of metallo-β-lactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp.

AU - Yong, Dongeun

AU - Lee, Kyungwon

AU - Yum, Jong Hwa

AU - Shin, Hee Bong

AU - Rossolini, Gian Maria

AU - Chong, Yunsop

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