Abstract
As a first step in the development of an optical biosensor for the detection of organophosphorus compounds, we describe the immobilization of conjugates of enzyme (AChE) and pH-sensitive fluorophore (SNAFL-1) within the PEG hydrogel microparticles via a dispersion photopolymerization. The fluorescent response of the PEG microparticles containing AChE-SNAFL conjugates showed that AChE-SNAFL conjugates were successfully encapsulated within the microparticles and that AChE was still active after the encapsulation procedure. Using a quantitative analysis of enzyme activity, we found that 70% of AChE activity was maintained after conjugation and encapsulation. A leaching test showed that there was no significant AChE leaching into the surrounding media. These results indicate that AChE-SNAFL conjugates could be encapsulated within PEG hydrogel microparticles without a significant loss of the enzyme activity and that these microparticles could potentially be used as optical biosensors for the detection of organophosphorus compounds.
| Original language | English |
|---|---|
| Journal | Current Applied Physics |
| Volume | 9 |
| Issue number | 4 SUPPL. |
| DOIs | |
| Publication status | Published - 2009 Jul 1 |
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All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Physics and Astronomy(all)
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Immobilization of enzymes within hydrogel microparticles to create optical biosensors for the detection of organophosphorus compounds. / Kim, Bumsang; Lee, Youngsik; Lee, Kangtaek; Koh, Won-Gun.
In: Current Applied Physics, Vol. 9, No. 4 SUPPL., 01.07.2009.Research output: Contribution to journal › Article
TY - JOUR
T1 - Immobilization of enzymes within hydrogel microparticles to create optical biosensors for the detection of organophosphorus compounds
AU - Kim, Bumsang
AU - Lee, Youngsik
AU - Lee, Kangtaek
AU - Koh, Won-Gun
PY - 2009/7/1
Y1 - 2009/7/1
N2 - As a first step in the development of an optical biosensor for the detection of organophosphorus compounds, we describe the immobilization of conjugates of enzyme (AChE) and pH-sensitive fluorophore (SNAFL-1) within the PEG hydrogel microparticles via a dispersion photopolymerization. The fluorescent response of the PEG microparticles containing AChE-SNAFL conjugates showed that AChE-SNAFL conjugates were successfully encapsulated within the microparticles and that AChE was still active after the encapsulation procedure. Using a quantitative analysis of enzyme activity, we found that 70% of AChE activity was maintained after conjugation and encapsulation. A leaching test showed that there was no significant AChE leaching into the surrounding media. These results indicate that AChE-SNAFL conjugates could be encapsulated within PEG hydrogel microparticles without a significant loss of the enzyme activity and that these microparticles could potentially be used as optical biosensors for the detection of organophosphorus compounds.
AB - As a first step in the development of an optical biosensor for the detection of organophosphorus compounds, we describe the immobilization of conjugates of enzyme (AChE) and pH-sensitive fluorophore (SNAFL-1) within the PEG hydrogel microparticles via a dispersion photopolymerization. The fluorescent response of the PEG microparticles containing AChE-SNAFL conjugates showed that AChE-SNAFL conjugates were successfully encapsulated within the microparticles and that AChE was still active after the encapsulation procedure. Using a quantitative analysis of enzyme activity, we found that 70% of AChE activity was maintained after conjugation and encapsulation. A leaching test showed that there was no significant AChE leaching into the surrounding media. These results indicate that AChE-SNAFL conjugates could be encapsulated within PEG hydrogel microparticles without a significant loss of the enzyme activity and that these microparticles could potentially be used as optical biosensors for the detection of organophosphorus compounds.
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U2 - 10.1016/j.cap.2009.06.043
DO - 10.1016/j.cap.2009.06.043
M3 - Article
VL - 9
JO - Current Applied Physics
JF - Current Applied Physics
SN - 1567-1739
IS - 4 SUPPL.
ER -