Immobilization of lipase on surface modified magnetic nanoparticles using alkyl benzenesulfonate

Kanagasabai Muruganandam Ponvel, Dong Geun Lee, Eun Ji Woo, Ik Sung Ahn, Chang Ha Lee

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The surface of nano-sized magnetite (NSM) particles synthesized by coprecipitation method was modified by alkyl benzenesulfonate (ABS) as a coating material. ABS on the NSM was expected to form a spacer between the surface of the NSM particles and the enzyme adsorbed and to play a role of strong enzyme adsorption onto a hydrophobic surface. Transmission electron microscopy showed that the NSM particles had an average size of 10 nm. Magnetic measurement revealed that the nanoparticles were superparamagnetic and the saturation magnetization was about 68 emu/g. Porcine pancreas lipase (PPL) was immobilized onto the ABS-NSM, which was to catalyze hydrolysis of olive oil and showed enhanced durability in the reuse after being recovered by magnetic separations.

Original languageEnglish
Pages (from-to)127-130
Number of pages4
JournalKorean Journal of Chemical Engineering
Volume26
Issue number1
DOIs
Publication statusPublished - 2009 Jan 1

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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