Immobilization of starch-converting enzymes on surface-modified carriers using single and co-immobilized systems: Properties and application to starch hydrolysis

Daewon Park, Seungjoo Haam, Kyongho Jang, Ik Sung Ahn, Woo Sik Kim

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Starch-converting enzymes, α-amylase and glucoamylase, were immobilized on surface-modified carriers using a co-immobilized as well as a single system. Hydrophilic silica gel and DEAE-cellulose entrapped in alginate beads were selected as the most effective carriers. The hydrophilic and the hydrophobic characteristics of carriers were made by polyethylimine-coating and silanization, respectively, and confirmed by contact angle measurements. All co-enzymes immobilized on hydrophilic carriers exhibited the highest activities in each modulation. The lower Km values (0.102 mg/ml for hydrophilic silica gel and 0.099 mg/ml for DEAE-cellulose entrapped in beads) than that of native enzyme (0.199 mg/ml) represented the higher affinity of immobilized enzymes to substrates. When performing the hydrolysis of starch, the shifts of optimum temperature from 50 to 60°C (or activation energy) and pH from 5.0 to 5.5 after immobilization were due to covalent bond formation and polyionic characteristics of carriers, respectively. The co-enzymes immobilized on hydrophilic silica gel and DEAE-cellulose entrapped in alginate beads exhibited 92.3 and 88.9% of the remaining activity even after 10 times of reuse.

Original languageEnglish
Pages (from-to)53-61
Number of pages9
JournalProcess Biochemistry
Volume40
Issue number1
DOIs
Publication statusPublished - 2005 Jan 1

Fingerprint

DEAE-Cellulose
Immobilized Enzymes
Silica Gel
Starch
Immobilization
Hydrolysis
Enzymes
Silica gel
Cellulose
Alginate
Glucan 1,4-alpha-Glucosidase
Covalent bonds
Amylases
Angle measurement
Contact angle
Activation energy
Modulation
Coatings
Temperature
Substrates

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

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title = "Immobilization of starch-converting enzymes on surface-modified carriers using single and co-immobilized systems: Properties and application to starch hydrolysis",
abstract = "Starch-converting enzymes, α-amylase and glucoamylase, were immobilized on surface-modified carriers using a co-immobilized as well as a single system. Hydrophilic silica gel and DEAE-cellulose entrapped in alginate beads were selected as the most effective carriers. The hydrophilic and the hydrophobic characteristics of carriers were made by polyethylimine-coating and silanization, respectively, and confirmed by contact angle measurements. All co-enzymes immobilized on hydrophilic carriers exhibited the highest activities in each modulation. The lower Km values (0.102 mg/ml for hydrophilic silica gel and 0.099 mg/ml for DEAE-cellulose entrapped in beads) than that of native enzyme (0.199 mg/ml) represented the higher affinity of immobilized enzymes to substrates. When performing the hydrolysis of starch, the shifts of optimum temperature from 50 to 60°C (or activation energy) and pH from 5.0 to 5.5 after immobilization were due to covalent bond formation and polyionic characteristics of carriers, respectively. The co-enzymes immobilized on hydrophilic silica gel and DEAE-cellulose entrapped in alginate beads exhibited 92.3 and 88.9{\%} of the remaining activity even after 10 times of reuse.",
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AU - Kim, Woo Sik

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