In this work, Pt nanoparticles were synthesised according to the citrate reduction process. The physical properties of these Pt nanoparticles were characterised using dynamic laser scattering (DLS), zeta-potential analysis, thermal gravimetric analysis (TGA), and TEM. The peroxidase activity of the Pt nanoparticles was also analysed using a chromogenic substrate (TMB) and a luminescent probe (luminol). For application to immunoassays, Pt nanoparticles were coupled with antibodies via electrostatic interaction, and the coupling conditions were optimised at different pH conditions. Finally, the applicability of Pt nanoparticles coupled with anti-hCG (human chorionic gonadotropin) antibodies was demonstrated for the immunoassay of hCG for the medical diagnosis of pregnancy.
Bibliographical noteFunding Information:
This work was supported by Defense Acquisition Program Administration (DAPA) and Agency for Defense Development (ADD) of South Korea. This research was supported by the National Research Foundation of South Korea (2012R1A2A2A03047461, 2011-0020285, 2014M3A9E5073818 and 2013R1A1A2065723). This work was also supported by the Creative Fusion Research Program through the Creative Allied Project funded by the Korea Research Council of Fundamental Science and Technology (CAP-12-1).
All Science Journal Classification (ASJC) codes
- Condensed Matter Physics
- Electrical and Electronic Engineering
- Materials Chemistry