Immunohistochemical distribution of toll-like receptor 4 in preterm human fetal membrane

Seong Jin Choi, Soon Hee Jung, Minseob Eom, Kyoung Hee Han, In Bai Chung, Soo Ki Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Aim: Toll-like receptor 4 (TLR-4) is the major receptor used for recognition of specific gram negative bacteria by the host immune system. The role of TLR-4 has been revealed in preterm parturition. This study aims to demonstrate the immunohistochemical expression of TLR-4 with regard to histological layers and anatomical regions of the human fetal membranes. Material and Methods: Fetal membranes were obtained from the uterine fundus and low segment. Immunohistochemical staining for TLR-4 and hematoxylin and eosin stain were performed. Results: The chorion expressed significantly higher levels of TLR-4 than the amnion (P = 0.001). There was no difference in the expression of TLR-4 between the uterine fundus and the uterine low segment (P = 0.942). There was no significant difference in TLR-4 expression according to the presence of histological chorioamnionitis (P = 0.444). TLR-4 expression decreased significantly with the progression of gestation (P = 0.002). Conclusions: The level of expression of TLR-4 did not differ according to anatomic location, but did differ according to the histological layer of the human fetal membranes and gestational age. These results suggest that TLR-4 may be involved in preterm parturition.

Original languageEnglish
Pages (from-to)108-112
Number of pages5
JournalJournal of Obstetrics and Gynaecology Research
Volume38
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1

Fingerprint

Extraembryonic Membranes
Toll-Like Receptor 4
Gestational Age
Parturition
Chorioamnionitis
Chorion
Amnion
Hematoxylin
Eosine Yellowish-(YS)
Gram-Negative Bacteria
Immune System
Coloring Agents
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynaecology

Cite this

Choi, Seong Jin ; Jung, Soon Hee ; Eom, Minseob ; Han, Kyoung Hee ; Chung, In Bai ; Kim, Soo Ki. / Immunohistochemical distribution of toll-like receptor 4 in preterm human fetal membrane. In: Journal of Obstetrics and Gynaecology Research. 2012 ; Vol. 38, No. 1. pp. 108-112.
@article{a31c766a83144f6db06f5f7d53bc8d9e,
title = "Immunohistochemical distribution of toll-like receptor 4 in preterm human fetal membrane",
abstract = "Aim: Toll-like receptor 4 (TLR-4) is the major receptor used for recognition of specific gram negative bacteria by the host immune system. The role of TLR-4 has been revealed in preterm parturition. This study aims to demonstrate the immunohistochemical expression of TLR-4 with regard to histological layers and anatomical regions of the human fetal membranes. Material and Methods: Fetal membranes were obtained from the uterine fundus and low segment. Immunohistochemical staining for TLR-4 and hematoxylin and eosin stain were performed. Results: The chorion expressed significantly higher levels of TLR-4 than the amnion (P = 0.001). There was no difference in the expression of TLR-4 between the uterine fundus and the uterine low segment (P = 0.942). There was no significant difference in TLR-4 expression according to the presence of histological chorioamnionitis (P = 0.444). TLR-4 expression decreased significantly with the progression of gestation (P = 0.002). Conclusions: The level of expression of TLR-4 did not differ according to anatomic location, but did differ according to the histological layer of the human fetal membranes and gestational age. These results suggest that TLR-4 may be involved in preterm parturition.",
author = "Choi, {Seong Jin} and Jung, {Soon Hee} and Minseob Eom and Han, {Kyoung Hee} and Chung, {In Bai} and Kim, {Soo Ki}",
year = "2012",
month = "1",
day = "1",
doi = "10.1111/j.1447-0756.2011.01626.x",
language = "English",
volume = "38",
pages = "108--112",
journal = "Journal of Obstetrics and Gynaecology Research",
issn = "1341-8076",
publisher = "Blackwell Publishing Asia",
number = "1",

}

Immunohistochemical distribution of toll-like receptor 4 in preterm human fetal membrane. / Choi, Seong Jin; Jung, Soon Hee; Eom, Minseob; Han, Kyoung Hee; Chung, In Bai; Kim, Soo Ki.

In: Journal of Obstetrics and Gynaecology Research, Vol. 38, No. 1, 01.01.2012, p. 108-112.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Immunohistochemical distribution of toll-like receptor 4 in preterm human fetal membrane

AU - Choi, Seong Jin

AU - Jung, Soon Hee

AU - Eom, Minseob

AU - Han, Kyoung Hee

AU - Chung, In Bai

AU - Kim, Soo Ki

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Aim: Toll-like receptor 4 (TLR-4) is the major receptor used for recognition of specific gram negative bacteria by the host immune system. The role of TLR-4 has been revealed in preterm parturition. This study aims to demonstrate the immunohistochemical expression of TLR-4 with regard to histological layers and anatomical regions of the human fetal membranes. Material and Methods: Fetal membranes were obtained from the uterine fundus and low segment. Immunohistochemical staining for TLR-4 and hematoxylin and eosin stain were performed. Results: The chorion expressed significantly higher levels of TLR-4 than the amnion (P = 0.001). There was no difference in the expression of TLR-4 between the uterine fundus and the uterine low segment (P = 0.942). There was no significant difference in TLR-4 expression according to the presence of histological chorioamnionitis (P = 0.444). TLR-4 expression decreased significantly with the progression of gestation (P = 0.002). Conclusions: The level of expression of TLR-4 did not differ according to anatomic location, but did differ according to the histological layer of the human fetal membranes and gestational age. These results suggest that TLR-4 may be involved in preterm parturition.

AB - Aim: Toll-like receptor 4 (TLR-4) is the major receptor used for recognition of specific gram negative bacteria by the host immune system. The role of TLR-4 has been revealed in preterm parturition. This study aims to demonstrate the immunohistochemical expression of TLR-4 with regard to histological layers and anatomical regions of the human fetal membranes. Material and Methods: Fetal membranes were obtained from the uterine fundus and low segment. Immunohistochemical staining for TLR-4 and hematoxylin and eosin stain were performed. Results: The chorion expressed significantly higher levels of TLR-4 than the amnion (P = 0.001). There was no difference in the expression of TLR-4 between the uterine fundus and the uterine low segment (P = 0.942). There was no significant difference in TLR-4 expression according to the presence of histological chorioamnionitis (P = 0.444). TLR-4 expression decreased significantly with the progression of gestation (P = 0.002). Conclusions: The level of expression of TLR-4 did not differ according to anatomic location, but did differ according to the histological layer of the human fetal membranes and gestational age. These results suggest that TLR-4 may be involved in preterm parturition.

UR - http://www.scopus.com/inward/record.url?scp=84863020398&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863020398&partnerID=8YFLogxK

U2 - 10.1111/j.1447-0756.2011.01626.x

DO - 10.1111/j.1447-0756.2011.01626.x

M3 - Article

C2 - 21827576

AN - SCOPUS:84863020398

VL - 38

SP - 108

EP - 112

JO - Journal of Obstetrics and Gynaecology Research

JF - Journal of Obstetrics and Gynaecology Research

SN - 1341-8076

IS - 1

ER -