Immunohistochemical Localization of Cytokeratins in the Junctional Region of Ectoderm and Endoderm

Akihiro Hosoya, Sungwook Kwak, Eun Jung Kim, Declan Patrick Lunny, E. Birgitte Lane, Sung Won Cho, Hansung Jung

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Although tridermic species have two junctional regions of ectoderm and endoderm between their epidermis and digestive tract, we actually know little about these particular boundaries. Cytokeratins are the major intermediate filaments of epithelial cells and show a high degree of tissue specificity. Therefore, to characterize the epithelial cells in the junctional region of ectoderm and endoderm, we immunohistochemically examined the localization of cytokeratins 5, 7/17, 14, 18, Sox17, and alpha-fetoprotein (AFP) in the oropharyngeal and anorectal regions during the mouse gastrulation process. At embryonic day (E) 9.5, cytokeratins 5, 7/17, 14, and 18 were detected in all epithelial cells of the oropharyngeal region. At E12.5, cytokeratin 5-positive cells were not observed in the middle area of the oral cavity; however, the immunoreactivity was strong in the anterior and posterior areas. The immunoreaction of cytokeratins 18 was seen only in the middle and posterior areas of the oral mucosa. Cytokeratins 7/17 and 14 were localized in all areas of the oropharyngeal region. Sox17 and AFP, which are endodermal markers, were detected in the middle and posterior areas of the oral mucosa, but not in the anterior area. Moreover, this same localization pattern of cytokeratins also existed in the anorectal region of the E12.5 embryo, suggesting that the localization of cytokeratins and endodermal markers might give an implication for the boundary between ectoderm and endoderm. These results also suggest that these cytokeratins are useful molecules for monitoring the epithelial cell differentiation in the junctional region of the germ layers. Anat Rec, 2010.

Original languageEnglish
Pages (from-to)1864-1872
Number of pages9
JournalAnatomical Record
Volume293
Issue number11
DOIs
Publication statusPublished - 2010 Nov 1

Fingerprint

Endoderm
Ectoderm
Keratin-5
Keratin-7
Keratins
epithelial cells
Epithelial Cells
digestive tract mucosa
alpha-Fetoproteins
Mouth Mucosa
Keratin-17
Keratin-14
Keratin-18
Germ Layers
intermediate filaments
Gastrulation
Organ Specificity
Intermediate Filaments
epidermis (animal)
Epidermis

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Biotechnology
  • Histology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Hosoya, A., Kwak, S., Kim, E. J., Lunny, D. P., Birgitte Lane, E., Cho, S. W., & Jung, H. (2010). Immunohistochemical Localization of Cytokeratins in the Junctional Region of Ectoderm and Endoderm. Anatomical Record, 293(11), 1864-1872. https://doi.org/10.1002/ar.21233
Hosoya, Akihiro ; Kwak, Sungwook ; Kim, Eun Jung ; Lunny, Declan Patrick ; Birgitte Lane, E. ; Cho, Sung Won ; Jung, Hansung. / Immunohistochemical Localization of Cytokeratins in the Junctional Region of Ectoderm and Endoderm. In: Anatomical Record. 2010 ; Vol. 293, No. 11. pp. 1864-1872.
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Hosoya, A, Kwak, S, Kim, EJ, Lunny, DP, Birgitte Lane, E, Cho, SW & Jung, H 2010, 'Immunohistochemical Localization of Cytokeratins in the Junctional Region of Ectoderm and Endoderm', Anatomical Record, vol. 293, no. 11, pp. 1864-1872. https://doi.org/10.1002/ar.21233

Immunohistochemical Localization of Cytokeratins in the Junctional Region of Ectoderm and Endoderm. / Hosoya, Akihiro; Kwak, Sungwook; Kim, Eun Jung; Lunny, Declan Patrick; Birgitte Lane, E.; Cho, Sung Won; Jung, Hansung.

In: Anatomical Record, Vol. 293, No. 11, 01.11.2010, p. 1864-1872.

Research output: Contribution to journalArticle

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AU - Hosoya, Akihiro

AU - Kwak, Sungwook

AU - Kim, Eun Jung

AU - Lunny, Declan Patrick

AU - Birgitte Lane, E.

AU - Cho, Sung Won

AU - Jung, Hansung

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Hosoya A, Kwak S, Kim EJ, Lunny DP, Birgitte Lane E, Cho SW et al. Immunohistochemical Localization of Cytokeratins in the Junctional Region of Ectoderm and Endoderm. Anatomical Record. 2010 Nov 1;293(11):1864-1872. https://doi.org/10.1002/ar.21233